HitHunter Kinase Enzyme Activity Assay Kits
Accurate determination of kinase enzyme activity is vital for supporting the development of safer and more effective therapeutics. The HitHunter® Kinase Enzyme Activity Assay Kits offer validated, ready-to-use platforms featuring high specificity and sensitivity, enabling efficient screening of serine/threonine, tyrosine, and lipid kinases in both low- and high- throughput formats. With red-shifted fluorescent detection, minimal compound interference, and the ability to tolerate high ATP concentrations (up to 1 mM), these assays streamline the measurement of ADP a universal byproduct of kinase mediated phosphorylation accelerating research on potential kinase inhibitors or activators.
Key Features & Benefits of HitHunter Kinase Enzyme Activity Assay Kits
Complete Ready-to-Use Kits - Each kit provides the kinase of interest, its specific substrate, detection reagents, assay buffer, ATP Gold reagent, plates, and comprehensive protocols for a hassle-free setup.
Non-Radioactive, Homogeneous Assay - The antibody-free, no-wash format measures ADP accumulation from the kinase-mediated transfer of a phosphate group from ATP to its substrate, using a fluorescence gain-of-signal readout that minimizes background
interference.
High Sensitivity & Wide Dynamic Range - Designed to work with minimal amounts of kinase per well, the assays deliver high signal-to-noise ratios and can tolerate ATP concentrations up to 1 mM—ideal for kinases with high Km ATP values or for replicating physiological conditions.
Scalable Throughput - Optimized for use in 384-and higher-well plate formats, these kits are suitable for both focused studies and large-scale high-throughput screening campaigns.
Rapid & Reproducible Workflow - Featuring a streamlined process with a 60-minute kinase reaction followed by a 30-minute incubation with detection reagents, the kits have been rigorously validated to ensure consistent and reproducible results.
Custom Development Services - We offer tailored enzyme activity assay kit development to support your unique drug discovery initiatives.
HitHunter Kinase Enzyme Activity Assay Principle
The HitHunter Kinase Enzyme Activity Assays rely on a two-step, ADP-based detection method that measures kinase-mediated phosphate transfer from ATP to a specific substrate. The ADP produced is converted into hydrogen peroxide, which, in the presence of peroxidase
and a fluorescent dye precursor (ADHP), generates a red-shifted fluorophore, resorufin (Figure 1). A stop solution stabilizes both signal and background, facilitating reliable and consistent data acquisition within approximately 90 minutes.
This gain-of-signal, non-radioactive approach is optimized for high throughput workflows, enabling rapid evaluation of kinase activity and potential inhibitors (Figure 2). This method has been extensively optimized for precision and reproducibility, ensuring robust performance in
diverse experimental settings.
Figure 1. HitHunter Kinase Enzyme Activity Assay Kit Assay Principle
HitHunter Kinase Enzyme Activity Assay Workflow
Figure 2. Schematic of the HitHunter Kinase Enzyme Activity Assay steps, from initial reagent preparation through final fluorescence detection.
HitHunter Kinase Enzyme Activity Assay Principle
Our kinase assay kits are trusted by discovery teams worldwide for measuring kinase activity, evaluating inhibitor potency (IC₅₀), and assessing ATP/substrate competition (Km, Ki). With applications spanning target validation, lead screening, structure-activity relationship (SAR) studies, and resistance modeling, these assays are engineered to integrate seamlessly into diverse R&D workflows. Whether you are performing primary HTS campaigns or refining inhibitor selectivity our platform ensures scientific rigor, operational efficiency, and maximum flexibility. Explore our kinase assay kits or Contact Us to develop a custom assay for your target of interest. Let us help you streamline your kinase discovery strategy from early screening to mechanistic insight.
Secure and boost
your discovery programs
Starting from native material or recombinant systems, we succeed with all types of proteins: Kinases, Phosphatases, Ubiquitins, Epigenetic Proteins, GPCRs, Ion Channels, Transporters, Receptors and Viral Proteins.
