Custom Protein Expression

Various cell expression systems are employed to synthesize proteins, each offering unique advantages. Understanding the intricacies of these systems is vital for optimizing the expression of proteins, especially challenging membrane proteins. Boosting your discovery programs with our tailored and cutting-edge solution.

Custom protein expression excellence: Eurofins CALIXAR's tailored solutions for biotechnology and drug discovery

Protein expression is a fundamental process in the realm of biotechnology and drug discovery. It involves the synthesis of proteins from their respective genes, often critical for understanding biological functions and the development of therapeutic solutions.

Eurofins CALIXAR's Custom Protein Expression Service leverages state-of-the-art technology to achieve the expression of native and functional proteins. We expertly adapt expression conditions to suit the specific target, offering a tailored approach for scientists, researchers, and teams in pharmaceutical, biotechnology, and academic settings. Eurofins CALIXAR excels in producing native and functional proteins for various applications, including antibody development, vaccine formulation, and structure-based drug design. Our unique advantage lies in our ability to adapt expression conditions according to the target, ensuring success in even the most complex projects.

Tailoring success with diverse protein expression systems

The choice of the cell system plays a crucial role in determining the success of your project.

Our Protein Expression Technology empowers the successful expression of membrane proteins in a variety of cell systems, including bacteria, yeast, insect cells, and mammalian cells.

Bacteria: powerhouse of prokaryotic expression

E. coli, a prokaryotic expression system, offers rapid and cost-effective protein production. It facilitates high yields, ideally for smaller proteins, especially those not requiring complex post-translational modifications.

Yeast: balancing simplicity and complexity

Yeast systems, including Saccharomyces cerevisiae, offer eukaryotic advantages, facilitating the expression of glycosylated proteins crucial for certain applications. This system strikes a balance between simplicity and post-translational modifications. Eurofins CALIXAR leverages this system for its adaptability to membrane proteins, ensuring native functionality.

Insect cells: mimicking complexity

Employed in baculovirus expression systems, the Sf9 insect cell expression system mimics eukaryotic complexity, offering a more native environment for membrane protein expression. It proves beneficial for complex proteins requiring intricate folding and post-translational modifications. Our expertise in this system ensures reliable expression and functionality.

Mammalian expression: precision and versatility

Human Embryonic Kidney (HEK) cells, a eukaryotic system, closely emulate the human cellular environment, and also offer precision and versatility. Ideal for expressing human membrane proteins, CALIXAR utilizes HEK cells to achieve precise folding and post-translational modifications, producing native and functional targets.

Chinese Hamster Ovary (CHO) cells, another eukaryotic system, excel in producing complex proteins with accurate glycosylation patterns. CALIXAR harnesses the precision of CHO cells to express membrane proteins, ensuring the production of native and functional targets.

Expression Systems
Cell lines
Major Applications

E. coli

·   the expression of small to medium-sized proteins.

·   the production of recombinant proteins for research purposes.

·   high-throughput expression screening

·   Rapid growth and high cell density.

·   Simple and cost-effective culture conditions.

·   Limited ability to express complex eukaryotic proteins.

·   Often forms inclusion bodies, making refolding necessary for obtaining soluble proteins.

·   Limited post-translational modifications.


S. cerevisiae, P. pastoris

·   Suitable for producing both soluble and membrane proteins, especially those requiring eukaryotic post-translational modifications.

·   S. cerevisiae is fast-growing and easy to manipulate genetically.

·   P. pastoris allows for controlled and inducible expression, preferred for membrane protein expression.

·   S. cerevisiae is fast-growing and easy to manipulate genetically.

·   P. pastoris allows for controlled and inducible expression.

Baculovirus - Insect cells

Sf9, Sf21

·   expressing complex eukaryotic proteins, including post-translationally modified proteins.

·   Capable of producing large and complex proteins.

·   Proper folding and post-translational modifications (e.g., glycosylation).

· Time-consuming compared to bacterial systems.

·  Higher cost due to the need for insect cell culture.



·   Widely used for producing therapeutic proteins, antibodies, and complex proteins with proper post-translational modifications.

·   Capable of producing proteins with human-like glycosylation and other post-translational modifications.

·   Suitable for large-scale production in biopharmaceutical applications.

·   Longer production times compared to other systems.

·   Higher cost due to the need for serum-free media and complex culture conditions.

Unlocking success with Eurofins CALIXAR's Technology

Eurofins CALIXAR's Membrane Protein Expression Technology, anchored in our eight patent families and over 28 publications, ensures success in expressing full-length membrane therapeutic targets. By adapting expression conditions to each target, we guarantee the production of high-quality, native, and functional proteins. Partner with Eurofins CALIXAR to unlock the potential of diverse expression systems and propel your bio-drug discovery programs to new heights.

Secure and boost
your discovery programs

Starting from native material or recombinant systems, we succeed with all types of proteins: Kinases, Phosphatases, Ubiquitins, Epigenetic Proteins, GPCRs, Ion Channels, Transporters, Receptors and Viral Proteins.