Protein purification is the accurate process of isolating a specific protein or set of proteins from a complex mixture. In the context of custom protein production, it is the gateway to obtaining proteins tailored to your unique specifications. This involves carefully extracting, separating, and refining proteins to meet the desired quality and purity standards.
Eurofins CALIXAR offers cutting-edge methods in protein purification
1. Immobilized Metal Affinity Chromatography (IMAC)
IMAC involves the use of metal ions, such as nickel or cobalt, immobilized on a solid support. These metal ions have a strong affinity for certain amino acid sequences, such as histidine tags commonly added to recombinant proteins. During this step, the protein of interest selectively binds to the metal ions, allowing for efficient purification.
2. Immuno-affinity Chromatography
Affinity chromatography utilizes the specific interactions between a ligand and the protein of interest. This technique is highly selective, as the ligand is chosen to bind specifically to the target protein. The bound protein is then eluted, resulting in a highly purified sample.
3. Size Exclusion Chromatography (SEC)
SEC separates proteins based on their size. In this step, a column with porous beads is used, allowing smaller molecules to enter the beads and take longer paths, while larger molecules pass through more quickly. SEC is effective in removing impurities based on size, resulting in a purified protein sample.
4. Hydrophobic Interaction Chromatography (HIC)
HIC relies on the hydrophobic regions of proteins. The stationary phase of the chromatography column contains hydrophobic groups, and proteins with exposed hydrophobic regions interact with these groups. Adjusting salt concentration in the mobile phase helps elute the proteins based on their hydrophobicity.
5. Ion Exchange Chromatography (IEX)
IEX separates proteins based on their net charge. The stationary phase contains charged groups, and proteins are selectively bound and eluted based on their overall charge. By adjusting the pH or salt concentration, proteins can be separated according to their charge characteristics.
Unlock the full potential of your research with our custom protein services, specifically tailored to deliver stable, functional, and high-quality proteins. In the context of custom protein services, it is a crucial step to ensure the final product meets the desired quality and purity standards. Our protein purification expertise ensures the integrity of native full-length proteins, both soluble and membrane-bound.
Secure and boost
your discovery programs
Starting from native material or recombinant systems, we succeed with all types of proteins: Kinases, Phosphatases, Ubiquitins, Epigenetic Proteins, GPCRs, Ion Channels, Transporters, Receptors and Viral Proteins.