PI3K (p110alpha (E545K)/p85alpha)
The PI3K (p110alpha (E545K)/p85alpha) Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical platform for accurately measuring kinase inhibitor efficacy targeting the PI3K complex. The kit includes recombinant PI3K (p110α (E545K)/p85α), substrate, ATP, detection reagents, and assay plates—enabling precise quantification of ADP accumulation across two, six or twenty five384-well plate formats. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.
Biological information
The Kinase Enzyme Activity Assay Kit is a
biochemical assay designed to measure
kinase inhibitors using ADP Hunter assay technology, optimized for the PI3K
(p110alpha (E545K)/p85alpha) complex. It
contains all necessary
reagents—recombinant PI3K (p110alpha
(E545K)/p85alpha) complex (Eurofins #14-
783), substrate, ATP, detection reagents, and
assay plates—along with a Certificate of
Analysis that specifies the optimal reaction
conditions. Each kit provides enough
materials for two, six or twenty five 384-well
assay plates.
The E545K substitution is a somatic
mutation in p110alpha linked to tumors of
the colon and brain. Both in vitro and in vivo
studies indicate that this mutation confers
higher lipid kinase activity than the wild type
and can induce oncogenic transformation
(Kang S. et al., PNAS, 2005; 102:802-807 and
Zhao J.J. et al., PNAS, 2005; 102:18443-
18448).
Product specifications
Chemical data
The Kinase Enzyme Activity Assay Kit is a
biochemical assay designed to measure
kinase inhibitors using ADP Hunter assay technology, optimized for the PI3K
(p110alpha (E545K)/p85alpha) complex. It
contains all necessary
reagents—recombinant PI3K (p110alpha
(E545K)/p85alpha) complex (Eurofins #14-
783), substrate, ATP, detection reagents, and
assay plates—along with a Certificate of
Analysis that specifies the optimal reaction
conditions. Each kit provides enough
materials for two, six or twenty five 384-well
assay plates.
The E545K substitution is a somatic
mutation in p110alpha linked to tumors of
the colon and brain. Both in vitro and in vivo
studies indicate that this mutation confers
higher lipid kinase activity than the wild type
and can induce oncogenic transformation
(Kang S. et al., PNAS, 2005; 102:802-807 and
Zhao J.J. et al., PNAS, 2005; 102:18443-
18448).
Product specifications
Lipid Kinase Activity Assay Biological information
The Kinase Enzyme Activity Assay Kit is a
biochemical assay designed to measure
kinase inhibitors using ADP Hunter assay technology, optimized for the PI3K
(p110alpha (E545K)/p85alpha) complex. It
contains all necessary
reagents—recombinant PI3K (p110alpha
(E545K)/p85alpha) complex (Eurofins #14-
783), substrate, ATP, detection reagents, and
assay plates—along with a Certificate of
Analysis that specifies the optimal reaction
conditions. Each kit provides enough
materials for two, six or twenty five 384-well
assay plates.
The E545K substitution is a somatic
mutation in p110alpha linked to tumors of
the colon and brain. Both in vitro and in vivo
studies indicate that this mutation confers
higher lipid kinase activity than the wild type
and can induce oncogenic transformation
(Kang S. et al., PNAS, 2005; 102:802-807 and
Zhao J.J. et al., PNAS, 2005; 102:18443-
18448).
HMH; MCM; CCM4; CWS5; MCAP; PI3K;
CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-
alpha (p110alpha) / p85; AGM7; GRB1;
IMD36; p85-alpha (p85alpha)
Lipid Kinase Activity Assay Usage
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