Biological Assays

Eurofins CALIXAR’s technology maintains the structural and functional integrities of antigens yielding accurate recognition of specific antibodies, ideally for a variety of immunoassays.

Best-in-class proteins for biological assays

High-quality native, pure, stable and functional membrane protein targets compatible with all immunoassay processes.

High-quality antigens are the cornerstone of reliable immunoassays, ensuring accurate and robust results, which are crucial for advancing research and diagnostics in the fields of healthcare, biotechnology, and life sciences. Eurofins CALIXAR’s technology sustains the structural and functional integrities of the antigens, providing accurate identification of specific antibodies. 

We employ patented technology and customized methods for each client's immunoassay assays, such as ELISA, SPR, and Proteomics. These techniques empower drug discovery scientists (Pharma, biotechs, academics) to investigate deep into antibody-antigen interactions, unearth crucial biomarkers, and attain a profound comprehension of intricate biological systems. Their applications span diverse fields, including cancer immunology, immuno-monitoring, virology, diagnostics, and antibody development.

Enzyme Activity Assays

Purified proteins can be employed to develop enzyme activity assays, providing a means to assess the catalytic efficiency and substrate specificity of enzymes. These assays often involve monitoring changes in substrate concentration, product formation, or alterations in absorbance, fluorescence, or luminescence. The precise measurement of enzyme activity is crucial for understanding biochemical pathways, drug discovery, and optimizing industrial processes.

Receptor-Ligand Binding Assays

With purified proteins, receptor-ligand binding assays can be established to investigate interactions critical for cell signaling, immune responses, or drug development. These assays utilize labeled ligands or receptors to quantify binding events. Techniques such as surface plasmon resonance (SPR) or fluorescence-based methods can provide real-time, high-throughput data, enabling the characterization of binding kinetics and affinity.

Protein-Protein Interaction Studies

Purified proteins are pivotal for developing assays that explore protein-protein interactions (PPIs). Techniques such as co-immunoprecipitation, pull-down assays, and biophysical methods like surface plasmon resonance can be utilized to dissect the intricacies of PPIs. Understanding these interactions is crucial for delineating cellular pathways, signaling cascades, and potential therapeutic targets.

Functional Bioassays

Functional bioassays can be designed using purified proteins to assess biological responses triggered by specific molecules. Examples include cell-based assays utilizing recombinant proteins to modulate cellular processes or bioactivity assays that measure the physiological effects of purified proteins. These assays are instrumental in drug discovery and development, as they provide insights into the functional consequences of protein activities.

Inhibition and Activation Assays

Purified proteins are essential for developing assays that assess the inhibitory or activating effects of molecules. In drug discovery, these assays are crucial for identifying potential therapeutic compounds. Inhibition assays measure the ability of a substance to impede protein function, while activation assays assess the capacity to enhance specific protein activities, offering valuable information for drug screening and development.

Access to CALIXAR's immunoassay technology

All of our clients gain access to our proprietary technologies and know-how (8 patent families, more than 28 publications).

The CALIXAR® platform uses patented technologies only available for clients under specific agreements (license, co-development, and service).

You can leverage CALIXAR’s proprietary technology as well as other best-in-class membrane protein technology that are unobtainable in the market.

Secure and boost
your discovery programs

Starting from native material or recombinant systems, we succeed with all types of proteins: Kinases, Phosphatases, Ubiquitins, Epigenetic Proteins, GPCRs, Ion Channels, Transporters, Receptors and Viral Proteins.