PI3K (p110alpha (H1047R)/p85alpha)
The PI3K (p110alpha (H1047R)/p85alpha) Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical platform for accurately measuring kinase inhibitor efficacy targeting the PI3K complex. The kit includes recombinant PI3K (p110α (H1047R) /p85α), substrate, ATP, detection reagents, and assay plates—enabling precise quantification of ADP accumulation across two, six or twenty five 384-well plate formats. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.
Biological information
The Kinase Enzyme Activity Assay Kit is a
biochemical assay designed to measure
kinase inhibitors using ADP assay
technology, optimized for the PI3K (p110α
(H1047R)/p85α) complex. The kit contains all the necessary reagents—including the
recombinant PI3K (p110α (H1047R)/p85α)
complex (Eurofins #14-792), substrate, ATP,
detection reagents, and assay plates—along
with a Certificate of Analysis that details the
optimal reaction conditions for detecting
kinase inhibitors. Each kit provides sufficient
material to run reactions on two, six or
twenty five 384-well assay plates, enabling
high-sensitivity detection of p110alpha
H1047R kinase activity
The H1047R substitution is a somatic
mutation in p110α associated with tumors
of the colon, stomach, breast, and brain.
Both in vitro and in vivo studies have
demonstrated that this mutation confers
higher lipid kinase activity than the wild type
and can induce oncogenic transformation
(Samuels Y. et al., Science (2004); 304:554;
(Kang S. et al., PNAS, 2005; 102:802-807 and
Zhao J.J. et al., PNAS, 2005; 102:18443-
18448).
Product specifications
Chemical data
The Kinase Enzyme Activity Assay Kit is a
biochemical assay designed to measure
kinase inhibitors using ADP assay
technology, optimized for the PI3K (p110α
(H1047R)/p85α) complex. The kit contains all the necessary reagents—including the
recombinant PI3K (p110α (H1047R)/p85α)
complex (Eurofins #14-792), substrate, ATP,
detection reagents, and assay plates—along
with a Certificate of Analysis that details the
optimal reaction conditions for detecting
kinase inhibitors. Each kit provides sufficient
material to run reactions on two, six or
twenty five 384-well assay plates, enabling
high-sensitivity detection of p110alpha
H1047R kinase activity
The H1047R substitution is a somatic
mutation in p110α associated with tumors
of the colon, stomach, breast, and brain.
Both in vitro and in vivo studies have
demonstrated that this mutation confers
higher lipid kinase activity than the wild type
and can induce oncogenic transformation
(Samuels Y. et al., Science (2004); 304:554;
(Kang S. et al., PNAS, 2005; 102:802-807 and
Zhao J.J. et al., PNAS, 2005; 102:18443-
18448).
Product specifications
Lipid Kinase Activity Assay Biological information
The Kinase Enzyme Activity Assay Kit is a
biochemical assay designed to measure
kinase inhibitors using ADP assay
technology, optimized for the PI3K (p110α
(H1047R)/p85α) complex. The kit contains all the necessary reagents—including the
recombinant PI3K (p110α (H1047R)/p85α)
complex (Eurofins #14-792), substrate, ATP,
detection reagents, and assay plates—along
with a Certificate of Analysis that details the
optimal reaction conditions for detecting
kinase inhibitors. Each kit provides sufficient
material to run reactions on two, six or
twenty five 384-well assay plates, enabling
high-sensitivity detection of p110alpha
H1047R kinase activity
The H1047R substitution is a somatic
mutation in p110α associated with tumors
of the colon, stomach, breast, and brain.
Both in vitro and in vivo studies have
demonstrated that this mutation confers
higher lipid kinase activity than the wild type
and can induce oncogenic transformation
(Samuels Y. et al., Science (2004); 304:554;
(Kang S. et al., PNAS, 2005; 102:802-807 and
Zhao J.J. et al., PNAS, 2005; 102:18443-
18448).
HMH; MCM; CCM4; CWS5; MCAP; PI3K;
CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-
alpha (p110alpha) / p85; AGM7; GRB1;
IMD36; p85-alpha (p85alpha)
Lipid Kinase Activity Assay Usage
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