Brought to you by:

PI3K (p110alpha/p85alpha)

The PI3K (p110alpha/p85alpha) Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical platform for accurately measuring kinase inhibitor efficacy targeting the PI3K complex. The kit includes recombinant PI3K (p110α/p85α), substrate, ATP, detection reagents, and assay plates—enabling precise quantification of ADP accumulation across two, six or twenty five 384-well plate formats. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.

Biological information

Background

The Kinase Enzyme Activity Assay Kit is a biochemical assay for the measurement of kinase inhibitors. The kit uses the HitHunter® assay technology and has been optimized for PI3K (p110alpha/p85alpha) complex. All reagents are provided in the kit, including recombinant PI3K (p110alpha/p85alpha) complex (Eurofins #14-602), substrate, ATP, detection reagents and assay plates. The COA that accompanies the kit details the optimal reaction conditions for the detection of kinase inhibitors. The kit includes sufficient material to run reactions for two 384-well assay plates.

Target class
Kinase Enzyme Activity Assay Kit
Family
PI3/PI4-kinase
Accession number
GenBank U79143.1 (p110alpha); GenBank XM_043865.4 (p85alpha)
Target Name
PI3K (p110alpha/p85alpha)
Target Alias
Phosphatidylinositol 4,5-bisphosphate 3- kinase catalytic subunit alpha isoform (p110alpha); PIK3CA / Phosphatidylinositol 3-kinase regulatory subunit alpha (p85alpha)
Origin
Human
Theori. MW
Affinity tag

Product specifications

Expression system
Purity
Purification method
Sample Buffer
Specified activity
Application
Storage conditions
12 months at -70°C
Usage disclaimer

Chemical data

Background

The Kinase Enzyme Activity Assay Kit is a biochemical assay for the measurement of kinase inhibitors. The kit uses the HitHunter® assay technology and has been optimized for PI3K (p110alpha/p85alpha) complex. All reagents are provided in the kit, including recombinant PI3K (p110alpha/p85alpha) complex (Eurofins #14-602), substrate, ATP, detection reagents and assay plates. The COA that accompanies the kit details the optimal reaction conditions for the detection of kinase inhibitors. The kit includes sufficient material to run reactions for two 384-well assay plates.

Compound name
Kinase Enzyme Activity Assay Kit
Catalog number
44-602
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
Solubility structure

Lipid Kinase Activity Assay Biological information

Background

The Kinase Enzyme Activity Assay Kit is a biochemical assay for the measurement of kinase inhibitors. The kit uses the HitHunter® assay technology and has been optimized for PI3K (p110alpha/p85alpha) complex. All reagents are provided in the kit, including recombinant PI3K (p110alpha/p85alpha) complex (Eurofins #14-602), substrate, ATP, detection reagents and assay plates. The COA that accompanies the kit details the optimal reaction conditions for the detection of kinase inhibitors. The kit includes sufficient material to run reactions for two 384-well assay plates.

Target class
Kinase Enzyme Activity Assay Kit
Family
PI3/PI4-kinase
Subfamily
Class I PI3K
Protein Name
p110alpha/p85alpha complex
Protein Alias
Phosphatidylinositol 4,5-bisphosphate 3- kinase catalytic subunit alpha isoform (p110alpha); PIK3CA / Phosphatidylinositol 3-kinase regulatory subunit alpha (p85alpha)
Accession Number
GenBank U79143.1 (p110alpha); GenBank XM_043865.4 (p85alpha)
UniProt Number
P42336 (p110alpha)/P27986 (p85alpha)
Gene Name
PIK3CA (p110alpha)/PIK3R1 (p85alpha)
Gene ID
5290 (p110alpha)/5295 (p85alpha)
Gene Aliases

HMH; MCM; CCM4; CWS5; MCAP; PI3K;
CLAPO; CLOVE; MCMTC; PI3K-alpha; p110-
alpha (p110alpha) / p85; AGM7; GRB1;
IMD36; p85-alpha (p85alpha)

Target Species
Human

Lipid Kinase Activity Assay Usage

Product Type
Enzyme Activity Assay Kits
Application
Drug Discovery & Development
Storage Conditions
12 months at -70°C
Usage disclaimer

These products may be covered by issued US
and/or foreign patents, patent applications
and subject to a Limited Use Label License.
Please visit discoverx.com/license for a list
of products that are governed by limited use
label license terms and relevant patent and
trademark information.

Lipid Kinase Activity Assay Information

Assay Type
Enzymatic
Assay Measures
ADP Accumulation
Datasheets
44-602x Datasheet
File N°1
File N°2
Kinase Enzyme Activity Assay User Manual Rev 1
€ 1 400,00 EUR
Purchase from our distributor
Button