NEK1, active Human Recombinant Kinase Protein
N-terminal GST-tagged and C-terminal 6His tagged, recombinant, human NEK1 amino acids 1-505 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
No items found.
Biological information
Background
NEK1 (NIMA-related kinase 1) is a serine/threonine protein kinase that is involved in a variety of cellular processes, including DNA damage response, cell cycle progression, and cilia function. NEK1 has been shown to be involved in the regulation of DNA damage response, including repair of double-strand breaks, and mutations in NEK1 have been linked to several diseases, including cancer and neurodegenerative disorders. Research has suggested that NEK1 plays a critical role in maintaining the integrity of the primary cilium, which is a sensory organelle present on the surface of most mammalian cells. Dysfunction of primary cilia has been linked to a wide range of developmental and disease-related conditions, including polycystic kidney disease and retinal degeneration. NEK1 has also been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Target class
Kinase
Family
Accession number
NM_012224.2
Target Name
NEK1, active Human Recombinant Kinase Protein
Target Alias
Origin
Human
Theori. MW
86 kDa
Affinity tag
GST, 6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 47% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified using immobilized metal affinity
chromatography.
Sample Buffer
Protein in 50 mM Tris/HCl pH 7.5,
300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1
mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen
solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
NEK1 (NIMA-related kinase 1) is a serine/threonine protein kinase that is involved in a variety of cellular processes, including DNA damage response, cell cycle progression, and cilia function. NEK1 has been shown to be involved in the regulation of DNA damage response, including repair of double-strand breaks, and mutations in NEK1 have been linked to several diseases, including cancer and neurodegenerative disorders. Research has suggested that NEK1 plays a critical role in maintaining the integrity of the primary cilium, which is a sensory organelle present on the surface of most mammalian cells. Dysfunction of primary cilia has been linked to a wide range of developmental and disease-related conditions, including polycystic kidney disease and retinal degeneration. NEK1 has also been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Compound name
Kinase
Catalog number
15-020
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Kinase Activity Assay Biological information
Background
NEK1 (NIMA-related kinase 1) is a serine/threonine protein kinase that is involved in a variety of cellular processes, including DNA damage response, cell cycle progression, and cilia function. NEK1 has been shown to be involved in the regulation of DNA damage response, including repair of double-strand breaks, and mutations in NEK1 have been linked to several diseases, including cancer and neurodegenerative disorders. Research has suggested that NEK1 plays a critical role in maintaining the integrity of the primary cilium, which is a sensory organelle present on the surface of most mammalian cells. Dysfunction of primary cilia has been linked to a wide range of developmental and disease-related conditions, including polycystic kidney disease and retinal degeneration. NEK1 has also been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Target class
Kinase
Family
Subfamily
Protein Name
NEK1
Protein Alias
Accession Number
NM_012224.2
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
NEK1 (NIMA-related kinase 1) is a serine/threonine protein kinase that is involved in a variety of cellular processes, including DNA damage response, cell cycle progression, and cilia function. NEK1 has been shown to be involved in the regulation of DNA damage response, including repair of double-strand breaks, and mutations in NEK1 have been linked to several diseases, including cancer and neurodegenerative disorders. Research has suggested that NEK1 plays a critical role in maintaining the integrity of the primary cilium, which is a sensory organelle present on the surface of most mammalian cells. Dysfunction of primary cilia has been linked to a wide range of developmental and disease-related conditions, including polycystic kidney disease and retinal degeneration. NEK1 has also been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Target class
Kinase
Family
Accession Number
NM_012224.2
Target Name
NEK1
Target Alias
Origin
Human
Theoretical MW
Affinity Tag
GST, 6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 47% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified using immobilized metal affinity
chromatography.
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Biological Information
Background
NEK1 (NIMA-related kinase 1) is a serine/threonine protein kinase that is involved in a variety of cellular processes, including DNA damage response, cell cycle progression, and cilia function. NEK1 has been shown to be involved in the regulation of DNA damage response, including repair of double-strand breaks, and mutations in NEK1 have been linked to several diseases, including cancer and neurodegenerative disorders. Research has suggested that NEK1 plays a critical role in maintaining the integrity of the primary cilium, which is a sensory organelle present on the surface of most mammalian cells. Dysfunction of primary cilia has been linked to a wide range of developmental and disease-related conditions, including polycystic kidney disease and retinal degeneration. NEK1 has also been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Target class
Kinase
Family
Accession Number
NM_012224.2
Target Name
NEK1
Target Alias
Origin
Human
Theoretical MW
Affinity Tag
GST, 6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 47% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified using immobilized metal affinity
chromatography.
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only