CleavX HRV-3C Protease
CleavX HRV-3C Protease is a recombinant human rhinovirus 3C protease expressed in E. coli and purified by immobilized metal affinity chromatography (IMAC) to high purity (≥95%). The enzyme specifically recognizes and cleaves the LEVLFQ/GP motif with high catalytic efficiency, enabling precise removal of affinity or solubility tags from fusion proteins. The protease is supplied in a Tris-NaCl buffer containing glycerol and DTT, ensuring stability and preserving activity under standard storage and reaction conditions. Functional characterization demonstrates >95% cleavage of model substrates within 16 hours at 4 °C in PBS, making it well-suited for workflows that require low-temperature processing, such as preparation of proteins for structural or functional studies.
Biological information
6His and Strep tagged, HRV-3C protease in Tris-NaCl containing 10% glycerol and 1 mM DTT. The protease cuts the sequence LEVLFQ/GP on target proteins. 1 Unit of HRV 3C Protease cleaves 95% of 100 μg of test protein in 16 hours at 4°C in x PBS. The two affinity tags on the protease provide additional re-purification options for the cleaved target protein.
Product specifications
Chemical data
6His and Strep tagged, HRV-3C protease in Tris-NaCl containing 10% glycerol and 1 mM DTT. The protease cuts the sequence LEVLFQ/GP on target proteins. 1 Unit of HRV 3C Protease cleaves 95% of 100 μg of test protein in 16 hours at 4°C in x PBS. The two affinity tags on the protease provide additional re-purification options for the cleaved target protein.
Product specifications
Lipid Kinase Activity Assay Biological information
6His and Strep tagged, HRV-3C protease in Tris-NaCl containing 10% glycerol and 1 mM DTT. The protease cuts the sequence LEVLFQ/GP on target proteins. 1 Unit of HRV 3C Protease cleaves 95% of 100 μg of test protein in 16 hours at 4°C in x PBS. The two affinity tags on the protease provide additional re-purification options for the cleaved target protein.
Lipid Kinase Activity Assay Usage
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Please visit www.discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Lipid Kinase Activity Assay Information
Biological Information
6His and Strep tagged, HRV-3C protease in Tris-NaCl containing 10% glycerol and 1 mM DTT. The protease cuts the sequence LEVLFQ/GP on target proteins. 1 Unit of HRV 3C Protease cleaves 95% of 100 μg of test protein in 16 hours at 4°C in x PBS. The two affinity tags on the protease provide additional re-purification options for the cleaved target protein.
Product Specifications
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License.
Please visit www.discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Biological Information
6His and Strep tagged, HRV-3C protease in Tris-NaCl containing 10% glycerol and 1 mM DTT. The protease cuts the sequence LEVLFQ/GP on target proteins. 1 Unit of HRV 3C Protease cleaves 95% of 100 μg of test protein in 16 hours at 4°C in x PBS. The two affinity tags on the protease provide additional re-purification options for the cleaved target protein.
Product Specifications
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License.
Please visit www.discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.