CleavX HRV-3C Protease
CleavX HRV-3C Protease is a recombinant human rhinovirus 3C protease expressed in E. coli and purified by immobilized metal affinity chromatography (IMAC) to high purity (≥95%). The enzyme specifically recognizes and cleaves the LEVLFQ↓G/X motif with high catalytic efficiency, enabling precise removal of affinity or solubility tags from fusion proteins without leaving non-native residues. The protease is supplied in a Tris-NaCl buffer containing glycerol and DTT, ensuring stability and preserving activity under standard storage and reaction conditions. Functional characterization demonstrates >95% cleavage of model substrates within 16 hours at 4 °C in PBS, making it well-suited for workflows that require low-temperature processing, such as preparation of proteins for structural or functional studies.
Biological information
6His and Strep tagged, HRV 3C protease in
Tris-NaCl containing 10% glycerol and 1 mM
DTT. The protease cuts the sequence
LEVLFQ/GX on target proteins.
1 Unit of HRV 3C Protease cleaves >95% of
100 μg of test protein in 16 hours at 4°C in
1x PBS.
The two affinity tags on the protease
provide additional re-purification options for
the cleaved target protein.
Product specifications
Chemical data
6His and Strep tagged, HRV 3C protease in
Tris-NaCl containing 10% glycerol and 1 mM
DTT. The protease cuts the sequence
LEVLFQ/GX on target proteins.
1 Unit of HRV 3C Protease cleaves >95% of
100 μg of test protein in 16 hours at 4°C in
1x PBS.
The two affinity tags on the protease
provide additional re-purification options for
the cleaved target protein.
Product specifications
Lipid Kinase Activity Assay Biological information
6His and Strep tagged, HRV 3C protease in
Tris-NaCl containing 10% glycerol and 1 mM
DTT. The protease cuts the sequence
LEVLFQ/GX on target proteins.
1 Unit of HRV 3C Protease cleaves >95% of
100 μg of test protein in 16 hours at 4°C in
1x PBS.
The two affinity tags on the protease
provide additional re-purification options for
the cleaved target protein.
Lipid Kinase Activity Assay Usage
These products may be covered by issued US
and/or foreign patents, patent applications
and subject to a Limited Use Label License.
Please visit discoverx.com/license for a list
of products that are governed by limited use
label license terms and relevant patent and
trademark information.
Lipid Kinase Activity Assay Information
Biological Information
6His and Strep tagged, HRV 3C protease in
Tris-NaCl containing 10% glycerol and 1 mM
DTT. The protease cuts the sequence
LEVLFQ/GX on target proteins.
1 Unit of HRV 3C Protease cleaves >95% of
100 μg of test protein in 16 hours at 4°C in
1x PBS.
The two affinity tags on the protease
provide additional re-purification options for
the cleaved target protein.
Product Specifications
These products may be covered by issued US
and/or foreign patents, patent applications
and subject to a Limited Use Label License.
Please visit discoverx.com/license for a list
of products that are governed by limited use
label license terms and relevant patent and
trademark information.
Biological Information
6His and Strep tagged, HRV 3C protease in
Tris-NaCl containing 10% glycerol and 1 mM
DTT. The protease cuts the sequence
LEVLFQ/GX on target proteins.
1 Unit of HRV 3C Protease cleaves >95% of
100 μg of test protein in 16 hours at 4°C in
1x PBS.
The two affinity tags on the protease
provide additional re-purification options for
the cleaved target protein.
Product Specifications
These products may be covered by issued US
and/or foreign patents, patent applications
and subject to a Limited Use Label License.
Please visit discoverx.com/license for a list
of products that are governed by limited use
label license terms and relevant patent and
trademark information.