CaMKK1, active Human Recombinant Kinase Protein
10 µg of active CaMKK1. N-terminal 6His-tagged, recombinant, human CaMKK1 full length.
No items found.
Biological information
Background
The Calcium/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Calcium/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions. CaMKK1 plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
OTHER-CAMKK
Accession number
NM_172206.1
Target Name
CaMKK1, active Human Recombinant Kinase Protein
Target Alias
CAMKKA; CaM-KK Alpha; EC 2.7.11.17; Calcium/Calmodulin Dependent Protein Kinase Kinase 1
Origin
Human
Theori. MW
60 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 96% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The Calcium/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Calcium/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions. CaMKK1 plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. [provided by RefSeq, Jul 2008]
Compound name
Kinase
Catalog number
15-022
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Kinase Activity Assay Biological information
Background
The Calcium/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Calcium/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions. CaMKK1 plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
OTHER-CAMKK
Subfamily
Protein Name
CaMKK1
Protein Alias
CAMKKA; CaM-KK Alpha; EC 2.7.11.17; Calcium/Calmodulin Dependent Protein Kinase Kinase 1
Accession Number
NM_172206.1
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
The Calcium/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Calcium/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions. CaMKK1 plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
OTHER-CAMKK
Accession Number
NM_172206.1
Target Name
CaMKK1
Target Alias
CAMKKA; CaM-KK Alpha; EC 2.7.11.17; Calcium/Calmodulin Dependent Protein Kinase Kinase 1
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 96% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Biological Information
Background
The Calcium/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Calcium/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions. CaMKK1 plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
OTHER-CAMKK
Accession Number
NM_172206.1
Target Name
CaMKK1
Target Alias
CAMKKA; CaM-KK Alpha; EC 2.7.11.17; Calcium/Calmodulin Dependent Protein Kinase Kinase 1
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 96% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only