Akt3/PKBgamma (S472D) Kinase Enzyme Activity Assay Kit
The AKT3 Kinase Enzyme Activity Assay Kit offers a validated, ready-to-use biochemical assay based on the renowned ADP Hunter™ Plus Assay platform from DiscoverX for accurately measuring kinase inhibitor efficacy. The kit includes recombinant PKB gamma, substrate, ATP, detection reagents, and assay plates, enabling precise quantification of ADP accumulation. Designed to handle elevated ATP concentrations, this assay delivers robust, reliable data ideal for high-throughput screening (HTS) and other drug discovery applications.
Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT3. It comes with all essential components, including recombinant AKT3 (Eurofins #14-502), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT3 is one of 3 closely related serine/threonine-protein kinases, AKT1, AKT2 and AKT3, called the AKT kinase, which functions as a core effector of the PI3K‑AKT‑mTOR signalling cascade regulating diverse cellular processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is highly expressed in neuronal cells and plays a particularly prominent role in the central nervous system and its development. Mutations in AKT3 lead to a spectrum of neurodevelopmental disorders including megalencephaly, hemimegalencephaly and polymicrogyria. Beyond neurodevelopment, AKT3 is implicated in cancer pathogenesis and progression with frequent overexpression in human cancers.
Product specifications
Chemical data
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT3. It comes with all essential components, including recombinant AKT3 (Eurofins #14-502), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT3 is one of 3 closely related serine/threonine-protein kinases, AKT1, AKT2 and AKT3, called the AKT kinase, which functions as a core effector of the PI3K‑AKT‑mTOR signalling cascade regulating diverse cellular processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is highly expressed in neuronal cells and plays a particularly prominent role in the central nervous system and its development. Mutations in AKT3 lead to a spectrum of neurodevelopmental disorders including megalencephaly, hemimegalencephaly and polymicrogyria. Beyond neurodevelopment, AKT3 is implicated in cancer pathogenesis and progression with frequent overexpression in human cancers.
Product specifications
Kinase Activity Assay Biological information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT3. It comes with all essential components, including recombinant AKT3 (Eurofins #14-502), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT3 is one of 3 closely related serine/threonine-protein kinases, AKT1, AKT2 and AKT3, called the AKT kinase, which functions as a core effector of the PI3K‑AKT‑mTOR signalling cascade regulating diverse cellular processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is highly expressed in neuronal cells and plays a particularly prominent role in the central nervous system and its development. Mutations in AKT3 lead to a spectrum of neurodevelopmental disorders including megalencephaly, hemimegalencephaly and polymicrogyria. Beyond neurodevelopment, AKT3 is implicated in cancer pathogenesis and progression with frequent overexpression in human cancers.
MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
Kinase Activity Assay Usage
For Research Use Only
Kinase Activity Assay Information
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT3. It comes with all essential components, including recombinant AKT3 (Eurofins #14-502), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT3 is one of 3 closely related serine/threonine-protein kinases, AKT1, AKT2 and AKT3, called the AKT kinase, which functions as a core effector of the PI3K‑AKT‑mTOR signalling cascade regulating diverse cellular processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is highly expressed in neuronal cells and plays a particularly prominent role in the central nervous system and its development. Mutations in AKT3 lead to a spectrum of neurodevelopmental disorders including megalencephaly, hemimegalencephaly and polymicrogyria. Beyond neurodevelopment, AKT3 is implicated in cancer pathogenesis and progression with frequent overexpression in human cancers.
Product Specifications
For Research Use Only
Biological Information
The Kinase Enzyme Activity Assay Kit provides a reliable biochemical method for evaluating kinase inhibitors. Built on HitHunter® assay technology, this kit is specifically optimized for AKT3. It comes with all essential components, including recombinant AKT3 (Eurofins #14-502), a substrate, ATP, detection reagents, and assay plates. Each kit is accompanied by a Certificate of Analysis (COA), which outlines the recommended reaction conditions for accurate inhibitor detection. The supplied materials are sufficient to perform experiments covering two, six or twenty five 384-well assay plates.
AKT3 is one of 3 closely related serine/threonine-protein kinases, AKT1, AKT2 and AKT3, called the AKT kinase, which functions as a core effector of the PI3K‑AKT‑mTOR signalling cascade regulating diverse cellular processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is highly expressed in neuronal cells and plays a particularly prominent role in the central nervous system and its development. Mutations in AKT3 lead to a spectrum of neurodevelopmental disorders including megalencephaly, hemimegalencephaly and polymicrogyria. Beyond neurodevelopment, AKT3 is implicated in cancer pathogenesis and progression with frequent overexpression in human cancers.
Product Specifications
For Research Use Only