XIAP, active
XIAP active. N-terminal GSTtagged, recombinant human XIAP full length, expressed by baculovirus in Sf21 insect cells.
No items found.
Biological information
Background
X-linked inhibitor of apoptosis (XIAP) is one of a group of intracellular proteins which have been shown to act as endogenous inhibitors of caspases, the enzymatic effectors of apoptosis. XIAP possesses a C-terminal RING domain that functions as an E3 ubiquitin ligase and is able to ubiquitinate a variety of substrates thereby affecting a broad range of cellular activities beyond apoptotic suppression. There is evidence that XIAP plays a significant role in cell division, morphogenesis, heavy metal homeostasis, NF-kB activation and MAP kinase signalling. XIAP expression has been shown to be elevated in several malignancies and so is considered a promising target in anticancer therapies. Expected Purity 89% by SDS-PAGE and Coomassie blue staining.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_001167
Target Name
XIAP, active
Target Alias
API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
Origin
Human
Theori. MW
83 kDa
Affinity tag
N-terminal GST-tag
Product specifications
Expression system
expressed in insect cells
Purity
89%
Purification method
Glutathione sepharose, followed by gel filtration
Sample Buffer
0.17mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
X-linked inhibitor of apoptosis (XIAP) is one of a group of intracellular proteins which have been shown to act as endogenous inhibitors of caspases, the enzymatic effectors of apoptosis. XIAP possesses a C-terminal RING domain that functions as an E3 ubiquitin ligase and is able to ubiquitinate a variety of substrates thereby affecting a broad range of cellular activities beyond apoptotic suppression. There is evidence that XIAP plays a significant role in cell division, morphogenesis, heavy metal homeostasis, NF-kB activation and MAP kinase signalling. XIAP expression has been shown to be elevated in several malignancies and so is considered a promising target in anticancer therapies. Expected Purity 89% by SDS-PAGE and Coomassie blue staining.
Compound name
Ubiquitin
Catalog number
23-056
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
X-linked inhibitor of apoptosis (XIAP) is one of a group of intracellular proteins which have been shown to act as endogenous inhibitors of caspases, the enzymatic effectors of apoptosis. XIAP possesses a C-terminal RING domain that functions as an E3 ubiquitin ligase and is able to ubiquitinate a variety of substrates thereby affecting a broad range of cellular activities beyond apoptotic suppression. There is evidence that XIAP plays a significant role in cell division, morphogenesis, heavy metal homeostasis, NF-kB activation and MAP kinase signalling. XIAP expression has been shown to be elevated in several malignancies and so is considered a promising target in anticancer therapies. Expected Purity 89% by SDS-PAGE and Coomassie blue staining.
Target class
Ubiquitin
Family
E3
Subfamily
Protein Name
XIAP
Protein Alias
API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3
Accession Number
GenBank NM_001167
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures