HIF-1a
N-Terminal c-Myc and GST-tagged, recombinant, human HIF-1a, amino acid residues 331-609, expressed by baculovirus in Sf21 insect cells.
Biological information
Background
HIF-1a plays a critical role in the cellular response to low oxygen levels, or hypoxia. HIF-1a is a transcription factor, which means it regulates the expression of genes by binding to specific DNA sequences in their promoters.
HIF-1a is involved in various pathological conditions, such as cancer, ischemia, and inflammation. In cancer, HIF-1a can promote tumor growth and metastasis by upregulating genes involved in angiogenesis and metabolism. Targeting HIF-1a signaling is therefore a potential therapeutic strategy for the treatment of cancer and other hypoxia-related diseases.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank NM_001530
Target Name
HIF-1a
Target Alias
HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Origin
Human
Theori. MW
60 kDa
Affinity tag
N-terminal c-Myc, GST-tag
Product specifications
Expression system
expressed in insect cells
Purity
47%
Purification method
Glutathione sepharose
Sample Buffer
0.72 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
HIF-1a plays a critical role in the cellular response to low oxygen levels, or hypoxia. HIF-1a is a transcription factor, which means it regulates the expression of genes by binding to specific DNA sequences in their promoters.
HIF-1a is involved in various pathological conditions, such as cancer, ischemia, and inflammation. In cancer, HIF-1a can promote tumor growth and metastasis by upregulating genes involved in angiogenesis and metabolism. Targeting HIF-1a signaling is therefore a potential therapeutic strategy for the treatment of cancer and other hypoxia-related diseases.
Compound name
Ubiquitin
Catalog number
23-045
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
23-045
Datasheet