HIF-1a
N-Terminal c-Myc and GST-tagged, recombinant, human HIF-1a, amino acid residues 331-609, expressed by baculovirus in Sf21 insect cells.
No items found.
Biological information
Background
HIF-1a plays a critical role in the cellular response to low oxygen levels, or hypoxia. HIF-1a is a transcription factor, which means it regulates the expression of genes by binding to specific DNA sequences in their promoters.
HIF-1a is involved in various pathological conditions, such as cancer, ischemia, and inflammation. In cancer, HIF-1a can promote tumor growth and metastasis by upregulating genes involved in angiogenesis and metabolism. Targeting HIF-1a signaling is therefore a potential therapeutic strategy for the treatment of cancer and other hypoxia-related diseases.
Target class
Ubiquitin
Family
Substrate
Accession number
GenBank NM_001530
Target Name
HIF-1a
Target Alias
HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Origin
Human
Theori. MW
60 kDa
Affinity tag
N-terminal c-Myc, GST-tag
Product specifications
Expression system
expressed in insect cells
Purity
47%
Purification method
Glutathione sepharose
Sample Buffer
0.72 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
HIF-1a plays a critical role in the cellular response to low oxygen levels, or hypoxia. HIF-1a is a transcription factor, which means it regulates the expression of genes by binding to specific DNA sequences in their promoters.
HIF-1a is involved in various pathological conditions, such as cancer, ischemia, and inflammation. In cancer, HIF-1a can promote tumor growth and metastasis by upregulating genes involved in angiogenesis and metabolism. Targeting HIF-1a signaling is therefore a potential therapeutic strategy for the treatment of cancer and other hypoxia-related diseases.
Compound name
Ubiquitin
Catalog number
23-045
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
HIF-1a plays a critical role in the cellular response to low oxygen levels, or hypoxia. HIF-1a is a transcription factor, which means it regulates the expression of genes by binding to specific DNA sequences in their promoters.
HIF-1a is involved in various pathological conditions, such as cancer, ischemia, and inflammation. In cancer, HIF-1a can promote tumor growth and metastasis by upregulating genes involved in angiogenesis and metabolism. Targeting HIF-1a signaling is therefore a potential therapeutic strategy for the treatment of cancer and other hypoxia-related diseases.
Target class
Ubiquitin
Family
Substrate
Subfamily
Protein Name
HIF-1a
Protein Alias
HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
Accession Number
GenBank NM_001530
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures