Smad7
N-Terminal c-Myc and 6His-tagged, recombinant, human Smad7, full length, expressed by baculovirus in Sf21 insect cells.
No items found.
Biological information
Background
Smad7 plays a key role in the TGF-β signaling pathway. It is a member of the Smad family of proteins, which are intracellular signaling molecules that transduce signals from TGF-β receptors to the nucleus. Smad7 functions as a negative regulator of TGF-β signaling by interfering with the activation of downstream effectors, including Smad2 and Smad3. Specifically, Smad7 can inhibit the phosphorylation of Smad2/3 by the TGF-β receptor, which is required for their activation and subsequent translocation to the nucleus to regulate gene expression.
Smad7 can also act as an E3 ubiquitin ligase, promoting the degradation of TGF-β receptor complexes. Smad7 can bind to the TGF-β receptor and recruit the E2 ubiquitin-conjugating enzyme UbcH7, leading to the ubiquitination and degradation of the receptor complex by the proteasome.
Target class
Ubiquitin
Family
E3
Accession number
GenBank NM_005904
Target Name
Smad7
Target Alias
CRCS3, MADH7, MADH8
Origin
Human
Theori. MW
51 kDa
Affinity tag
N-terminal c-Myc, 6His-tag
Product specifications
Expression system
expressed in insect cells
Purity
89%
Purification method
Immobilized metal affinity chromatography
Sample Buffer
0.895 mg/ml of enzyme in 50 mM Tris/HCl pH 8.0, 150 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Smad7 plays a key role in the TGF-β signaling pathway. It is a member of the Smad family of proteins, which are intracellular signaling molecules that transduce signals from TGF-β receptors to the nucleus. Smad7 functions as a negative regulator of TGF-β signaling by interfering with the activation of downstream effectors, including Smad2 and Smad3. Specifically, Smad7 can inhibit the phosphorylation of Smad2/3 by the TGF-β receptor, which is required for their activation and subsequent translocation to the nucleus to regulate gene expression.
Smad7 can also act as an E3 ubiquitin ligase, promoting the degradation of TGF-β receptor complexes. Smad7 can bind to the TGF-β receptor and recruit the E2 ubiquitin-conjugating enzyme UbcH7, leading to the ubiquitination and degradation of the receptor complex by the proteasome.
Compound name
Ubiquitin
Catalog number
23-039
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
Smad7 plays a key role in the TGF-β signaling pathway. It is a member of the Smad family of proteins, which are intracellular signaling molecules that transduce signals from TGF-β receptors to the nucleus. Smad7 functions as a negative regulator of TGF-β signaling by interfering with the activation of downstream effectors, including Smad2 and Smad3. Specifically, Smad7 can inhibit the phosphorylation of Smad2/3 by the TGF-β receptor, which is required for their activation and subsequent translocation to the nucleus to regulate gene expression.
Smad7 can also act as an E3 ubiquitin ligase, promoting the degradation of TGF-β receptor complexes. Smad7 can bind to the TGF-β receptor and recruit the E2 ubiquitin-conjugating enzyme UbcH7, leading to the ubiquitination and degradation of the receptor complex by the proteasome.
Target class
Ubiquitin
Family
E3
Subfamily
Protein Name
Smad7
Protein Alias
CRCS3, MADH7, MADH8
Accession Number
GenBank NM_005904
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures