MLK4, active
N-terminal GST-tagged recombinant, human MKL4 amino acids 114-420.
No items found.
Biological information
Background
MLK4 (Mixed-Lineage Kinase 4) is a serine/threonine protein kinase that belongs to the mixed lineage kinase family of MAP3Ks (mitogen-activated protein Kinase kinase kinases). MLK4 has been shown to activate several downstream signaling pathways, including the c-Jun N-terminal kinase (JNK) pathway, the p38 MAPK pathway, and the NF-κB pathway, and it has been suggested to play a role in the regulation of cell survival, apoptosis, and inflammation. Research has suggested that MLK4 may also be involved in the regulation of several other physiological processes, including cardiovascular function and cancer progression. MLK4 has been implicated in the development and progression of several types of cancer, including breast cancer, prostate cancer, and pancreatic cancer, and efforts to develop small-molecule inhibitors of the kinase are underway as potential therapies for these conditions.
Target class
Kinase
Family
TKL
Accession number
BC136649.1
Target Name
MLK4, active
Target Alias
Origin
Human
Theori. MW
61 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 54% by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
MLK4 (Mixed-Lineage Kinase 4) is a serine/threonine protein kinase that belongs to the mixed lineage kinase family of MAP3Ks (mitogen-activated protein Kinase kinase kinases). MLK4 has been shown to activate several downstream signaling pathways, including the c-Jun N-terminal kinase (JNK) pathway, the p38 MAPK pathway, and the NF-κB pathway, and it has been suggested to play a role in the regulation of cell survival, apoptosis, and inflammation. Research has suggested that MLK4 may also be involved in the regulation of several other physiological processes, including cardiovascular function and cancer progression. MLK4 has been implicated in the development and progression of several types of cancer, including breast cancer, prostate cancer, and pancreatic cancer, and efforts to develop small-molecule inhibitors of the kinase are underway as potential therapies for these conditions.
Compound name
Kinase
Catalog number
16-051
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-051
Datasheet