AAK1, active
10 µg of active AAK1. N-terminal 6His-tagged, recombinant fragment of human AAK1.
Biological information
Background
AAK1 (AP2 Associated Kinase 1) belongs to the SNF1 subfamily of serine/threonine protein kinases. This kinase is detected in brain, heart and liver, and plays an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of AAK1 are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. [provided by RefSeq, Aug 2020]
Target class
Kinase
Family
OTHER-NAK
Accession number
NM_080836.2
Target Name
AAK1, active
Target Alias
AP2 Associated Kinase 1; KIAA1048; AP2-Associated Protein Kinase; Adaptor-Associated Kinase 1
Origin
Human
Theori. MW
43 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 96% by SDS-PAGE and Coomassie blue staining
Purification method
Immobilised metal affinity chromatography
Sample Buffer
enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
AAK1 (AP2 Associated Kinase 1) belongs to the SNF1 subfamily of serine/threonine protein kinases. This kinase is detected in brain, heart and liver, and plays an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of AAK1 are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. [provided by RefSeq, Aug 2020]
Compound name
Kinase
Catalog number
16-043
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-043
Datasheet