AAK1, active Human Recombinant Kinase Protein
10 µg of active AAK1. N-terminal 6His-tagged, recombinant fragment of human AAK1.
No items found.
Biological information
Background
AAK1 (AP2 Associated Kinase 1) belongs to the SNF1 subfamily of serine/threonine protein kinases. This kinase is detected in brain, heart and liver, and plays an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of AAK1 are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. [provided by RefSeq, Aug 2020]
Target class
Kinase
Family
OTHER-NAK
Accession number
NM_080836.2
Target Name
AAK1, active Human Recombinant Kinase Protein
Target Alias
AP2 Associated Kinase 1; KIAA1048; AP2-Associated Protein Kinase; Adaptor-Associated Kinase 1
Origin
Human
Theori. MW
43 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 96% by SDS-PAGE and Coomassie blue staining
Purification method
Immobilised metal affinity chromatography
Sample Buffer
enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
AAK1 (AP2 Associated Kinase 1) belongs to the SNF1 subfamily of serine/threonine protein kinases. This kinase is detected in brain, heart and liver, and plays an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of AAK1 are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. [provided by RefSeq, Aug 2020]
Compound name
Kinase
Catalog number
16-043
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
AAK1 (AP2 Associated Kinase 1) belongs to the SNF1 subfamily of serine/threonine protein kinases. This kinase is detected in brain, heart and liver, and plays an important role in regulating the clathrin-mediated endocytosis of the rabies virus, facilitating infection. Inhibitors of AAK1 are being studied as candidate therapeutics to disrupt the entry of viruses, including SARS-CoV-2, into target cells. It is also involved in positive regulation of Notch pathway signaling in mammals. [provided by RefSeq, Aug 2020]
Target class
Kinase
Family
OTHER-NAK
Subfamily
Protein Name
AAK1
Protein Alias
AP2 Associated Kinase 1; KIAA1048; AP2-Associated Protein Kinase; Adaptor-Associated Kinase 1
Accession Number
NM_080836.2
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures