TTK, active
N-terminal GST-tagged, recombinant, human TTK full length, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.
No items found.
Biological information
Background
TTK is a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.
Target class
Kinase
Family
CMGC
Accession number
NM_003318.3
Target Name
TTK, active
Target Alias
Origin
Human
Theori. MW
124 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 76% by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose
Sample Buffer
1.12 mg/ml of enzyme in 50 mM Tris/HCl pH 8.0, 4 mM glutathione, 266 mM NaCl, 0.09 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 0.9 mM benzamidine, 0.2 mM PMSF, 0.09% 2- mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
TTK is a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.
Compound name
Kinase
Catalog number
15-014
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
TTK is a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.
Target class
Kinase
Family
CMGC
Subfamily
Protein Name
TTK
Protein Alias
Accession Number
NM_003318.3
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures