PKR, active
N-terminal GST-tagged and C-terminal 10His tagged recombinant, human PKR amino acids 252-end.
No items found.
Biological information
Background
Protein kinase RNA-regulated (PKR), an ubiquitous serine/threonine kinase that is induced by interferons, is a critical signal transducer implicated in different stress-induced signaling pathways including dsRNA-induced TLR3 signaling and LPS-induced TLR4 signaling. Upon stress stimulation, PKR is activated by autophosphorylation and subsequently phosphorylates the alpha-subunit of eukaryotic initiation factor 2 (eIF-2a) thereby inducing apoptosis. PKR mediates the activation of important transcription factors, such as NF-kB, and MAP kinases, such as p382, by interacting with the TAK1 complex.
GenBank NM_002759.1
Target class
Kinase
Family
Ser/Thr Kinase
Accession number
NM_002759.1
Target Name
PKR, active
Target Alias
Origin
Human
Theori. MW
64 kDa
Affinity tag
GST, 10His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 85% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilised metal affinity chromatography
Sample Buffer
Protein in 40mM Tris/HCl pH7.5, 120mM NaCl, 240mM imidazole, 0.08mM EGTA, 20% (v/v) glycerol, 0.8mM benzamidine, 0.16mM PMSF, 0.08% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Protein kinase RNA-regulated (PKR), an ubiquitous serine/threonine kinase that is induced by interferons, is a critical signal transducer implicated in different stress-induced signaling pathways including dsRNA-induced TLR3 signaling and LPS-induced TLR4 signaling. Upon stress stimulation, PKR is activated by autophosphorylation and subsequently phosphorylates the alpha-subunit of eukaryotic initiation factor 2 (eIF-2a) thereby inducing apoptosis. PKR mediates the activation of important transcription factors, such as NF-kB, and MAP kinases, such as p382, by interacting with the TAK1 complex.
GenBank NM_002759.1
Compound name
Kinase
Catalog number
14-955
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
Protein kinase RNA-regulated (PKR), an ubiquitous serine/threonine kinase that is induced by interferons, is a critical signal transducer implicated in different stress-induced signaling pathways including dsRNA-induced TLR3 signaling and LPS-induced TLR4 signaling. Upon stress stimulation, PKR is activated by autophosphorylation and subsequently phosphorylates the alpha-subunit of eukaryotic initiation factor 2 (eIF-2a) thereby inducing apoptosis. PKR mediates the activation of important transcription factors, such as NF-kB, and MAP kinases, such as p382, by interacting with the TAK1 complex.
GenBank NM_002759.1
Target class
Kinase
Family
Ser/Thr Kinase
Subfamily
Protein Name
PKR
Protein Alias
Accession Number
NM_002759.1
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures