TAO3, active
N-terminal 6His-tagged recombinant, human TAO3 amino acids 1-411; N47S.
No items found.
Biological information
Background
TAO3 is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. In response to DNA damage, TAO3 is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases.
GenBank NM_016281. The recombinant protein contains the amino acid substitution N47S with reference to NM-016281. This mutation is reported inGenBank BC002756 and BC7007185
Target class
Kinase
Family
STE
Accession number
NM_016281.3
Target Name
TAO3, active
Target Alias
TAOK3, JIK, hKFC-A, MAP3K18, DPK, KDS, DKFZp666H245, FLJ31808
Origin
Human
Theori. MW
50.2 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 84% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
TAO3 is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. In response to DNA damage, TAO3 is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases.
GenBank NM_016281. The recombinant protein contains the amino acid substitution N47S with reference to NM-016281. This mutation is reported inGenBank BC002756 and BC7007185
Compound name
Kinase
Catalog number
14-745
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
TAO3 is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. In response to DNA damage, TAO3 is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases.
GenBank NM_016281. The recombinant protein contains the amino acid substitution N47S with reference to NM-016281. This mutation is reported inGenBank BC002756 and BC7007185
Target class
Kinase
Family
STE
Subfamily
Protein Name
TAO3
Protein Alias
TAOK3, JIK, hKFC-A, MAP3K18, DPK, KDS, DKFZp666H245, FLJ31808
Accession Number
NM_016281.3
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures
Biological Information
Background
TAO3 is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. In response to DNA damage, TAO3 is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases.
GenBank NM_016281. The recombinant protein contains the amino acid substitution N47S with reference to NM-016281. This mutation is reported inGenBank BC002756 and BC7007185
Target class
Kinase
Family
STE
Accession Number
NM_016281.3
Target Name
TAO3
Target Alias
TAOK3, JIK, hKFC-A, MAP3K18, DPK, KDS, DKFZp666H245, FLJ31808
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 84% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Biological Information
Background
TAO3 is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. In response to DNA damage, TAO3 is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases.
GenBank NM_016281. The recombinant protein contains the amino acid substitution N47S with reference to NM-016281. This mutation is reported inGenBank BC002756 and BC7007185
Target class
Kinase
Family
STE
Accession Number
NM_016281.3
Target Name
TAO3
Target Alias
TAOK3, JIK, hKFC-A, MAP3K18, DPK, KDS, DKFZp666H245, FLJ31808
Origin
Human
Theoretical MW
Affinity Tag
6His
Product Specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 84% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only