TAO3, active
N-terminal 6His-tagged recombinant, human TAO3 amino acids 1-411; N47S.
No items found.
Biological information
Background
TAO3 is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. In response to DNA damage, TAO3 is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases.
GenBank NM_016281. The recombinant protein contains the amino acid substitution N47S with reference to NM-016281. This mutation is reported inGenBank BC002756 and BC7007185
Target class
Kinase
Family
STE
Accession number
NM_016281.3
Target Name
TAO3, active
Target Alias
TAOK3, JIK, hKFC-A, MAP3K18, DPK, KDS, DKFZp666H245, FLJ31808
Origin
Human
Theori. MW
50.2 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 84% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
TAO3 is a serine/threonine protein kinase that activates the p38/MAPK14 stress-activated MAPK cascade but inhibits the basal activity of the MAPK8/JNK cascade. The encoded protein is a member of the GCK subfamily of STE20-like kinases. In response to DNA damage, TAO3 is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases.
GenBank NM_016281. The recombinant protein contains the amino acid substitution N47S with reference to NM-016281. This mutation is reported inGenBank BC002756 and BC7007185
Compound name
Kinase
Catalog number
14-745
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-745
Datasheet