Custom Recombinant Protein Development Services
Fully functional, native proteins for drug discovery, antibody development & structural biology: from sequence design to large-scale protein production with seamless downstream integration.
+750 protein projects delivered since 2011, including highly challenging and unstable targets
PhD-level scientists guiding every stage, from construct design to advanced analytics & biological validation
Extended QC and cutting-edge biophysics including SPR and HT-Spectral Shift screening, and structural biology support
End-to-end services delivering reproducible, high quality and decision-ready datasets
Membrane and Soluble Protein Expertise, Built for Scientific Performance
Eurofins CALIXAR excels in expressing protein targets that are often considered too complex or risky by conventional providers, including low-expression constructs, hydrophobic sequences, and structurally intricate, multi-domain proteins.
For both membrane and soluble targets, we tailor every step of the protein production process to preserve native structure, biological activity, and application compatibility.Each protein is delivered as a validated, ready-to-use reagent, supported by comprehensivedocumentation and QC aligned with your experimental needs.
Membrane Proteins
We develop GPCRs, ion channels, transporters, and other transmembrane proteins using multiple formats such as detergents, nanodiscs, and proteoliposomes.
These systems are selected and validated to ensure proper folding, functional orientation, and downstream performance — from ligand binding to structural studies.
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Soluble Proteins
Kinases, helicases, phosphatases, epigenetic regulators, and other intracellular enzymes are produced in full-length, active forms that retain their structural domains and catalytic activity.
Delivered in stable, assay-ready formats, they integrate seamlessly into screening, immunization, or mechanistic workflows.
Eurofins CALIXAR overcomes protein development challenges through target-tailored approaches that deliver:
*including high-throughput screening (HTS), immunization protocols, structural studies using cryo-electron microscopy (cryo-EM), and more
With over 20 years of experience, we support pharmaceutical, biotech and academic teams in advancing discovery with reliable, reproducible protein tools right from the start.
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From Misfolded to Functional: Solving Complex Expression Challenges
Expressing complex proteins like membrane-bound or multi-domain targets often causes low yield, aggregation, or lost activity, but Calixar solves these with proprietary strategies that deliver functional proteins, protect downstream workflows, and accelerate your timelines with reliable screening and structural results.
Struggling with difficult targets? Let's design your protein development strategy.
Over 750 complex protein projects completed, including 300+ membrane proteins (GPCRs, ion channels) and 500+ soluble targets, delivering functional proteins for pharma and biotech discovery programs worldwide.
Two decades mastering low-expression GPCRs, unstable ion channels, and aggregation-prone targets with PhD-level scientists ensuring native conformation, biological activity, and seamless integration into your structural biology and screening workflows
Fully integrated pipeline: construct design, mammalian/insect expression, proprietary extraction, multi-step purification, and comprehensive QC. All under one roof for maximum control, speed, and reproducibility.
92% of customers achieve their protein milestones on first attempt. Rescued projects where competitors failed, delivering application-ready proteins that accelerate your discovery.
From Sequence to Functional Protein: Integrated and Customizable
Protein Construct Design
Protein constructs with custom tags/fusion align with your project goals.
Protein Expression
Cutting-edge technology ensures high-yield, optimal protein performance.
Protein Solubilization & Stabilization
Expert techniques enhance solubility and stability using proprietary reagents.
Protein Purification
State-of-the-art methods isolate proteins with efficiency and quality.
Protein Characterization
Comprehensive analysis provides insights into structural and functional attributes.
Large-Scale Protein Production
Trust us to scale up your protein target with quality and quantity in mind.
Protein Identification
Precision protein identification for unrivaled structural and functional insights.
How we work
Discover our PhD-led collaborative approach, milestone-driven timelines with seamless handoffs across protein science.
From initial sequence design through protein development, analytics, biophysics, and structural biology, our dedicated project teams deliver more than proteins, they deliver success. See exactly how we partner to accelerate your discovery timelines.
Frequently Asked Questions
Eurofins Calixar is specialized in the expression, purification, and qualification of the most complex membrane and soluble proteins, from GPCRs to kinases.
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