Our protein construct design expertise spans gene synthesis, codon optimization, vector engineering, promoter selection, affinity tag integration, and signal peptide design to maximize expression, streamline purification, and enhance protein functionality. By tailoring each construct to the target protein and expression system, we ensure efficient production of high-quality proteins for research, structural biology, and drug discovery applications

Eurofins CALIXAR delivers tailored protein expression solutions designed to produce native, functional proteins for the most demanding research and drug discovery programs. Leveraging a broad range of expression systems, including E. coli, yeast, insect, HEK, and CHO cells, we optimize expression conditions for each target to maximize yield, quality, and biological relevance. From soluble proteins to complex membrane targets, our expertise ensures reliable native expression.

Our membrane protein solubilization and stabilization process is designed to preserve native structure, functionality, and stability. Leveraging extensive detergent screening, stabilizing reagents, nanodisc technologies (MSP-based nanodiscs, SMALPs, amphipols), and proteoliposome reconstitution, we tailor solubilization strategies to each target. These advanced approaches enable the reliable development of high-quality membrane proteins in biologically relevant environments, supporting applications in biochemistry, biophysics, and structural biology,.

Our state-of-the-art purification methods enable the isolation of highly pure, structurally intact, and biologically active proteins. Utilizing immobilized metal affinity chromatography (IMAC), immuno-affinity chromatography, ion exchange chromatography (IEX), hydrophobic interaction chromatography (HIC), and size exclusion chromatography (SEC), we develop target-specific purification workflows for both soluble and membrane proteins. These robust, multi-step strategies ensure the recovery of native full-length proteins with the purity, homogeneity, and stability required for advanced biophysical characterization, structural biology, and drug discovery applications.

Our large-scale protein production platform is designed to deliver high-quality recombinant proteins with the scalability, consistency, and reproducibility required for advanced research and drug discovery programs. Leveraging optimized upstream processes in flasks and bioreactors, coupled with automated downstream purification workflows, we enable the robust production of both soluble and membrane proteins at milligram scale. Rigorous quality control, standardized operating procedures, and batch-specific certificates of analysis ensure the purity, integrity, and traceability of every protein preparation.