Our protein purity and quality assessment methods combines biochemical and biophysical analyses to evaluate purity, homogeneity, concentration, stability, and aggregation propensity. Using SDS-PAGE, Native PAGE, Western Blot, analytical SEC, DLS/SLS, and NanoDSF, we provide comprehensive characterization of protein integrity, monodispersity, thermal stability, and sample quality. Quantitative protein assessment is performed using UV280/A280, BCA, and Bradford assays, ensuring accurate determination of protein concentration.

Our buffer screening and formulation capabilities leverage systematic stability profiling and thermal shift analyses across a wide range of pH, salt, and buffer conditions to identify optimal environments for protein stability and integrity. We develop tailored formulations to enhance protein solubility, monodispersity, and long-term stability, with specialized expertise in membrane protein stabilization using detergents, nanodiscs, and amphipols.

Our advanced protein characterization expertise provides detailed insights into protein assembly, molecular weight, and sample homogeneity using complementary biophysical and analytical techniques, including analytical SEC (aSEC), Mass Spectrometry, and Mass Photometry. We assess oligomeric state, distinguish monodisperse from polydisperse populations, and accurately determine molecular weight, enabling comprehensive evaluation of protein quality, structural integrity, and assembly.

Our binding and activity confirmation capabilities combine ligand-binding studies and functional assays to validate target engagement, biological activity, and protein functionality. Using reference compounds, enzyme activity assays, bioassays, and specialized assay platforms, we ensure proteins meet the quality standards required for research and drug discovery applications.