Novel innovative detergent family for MP extraction and stabilization

February 20, 2019

This work between CALIXAR, the University of Avignon and the Faculty of Biology of the University of Kaiserslautern highlights novel hydrogenated diglucose detergents for membrane-protein extraction and stabilization (Guillet P. et al., 2019, Langmuir).

This publication reports the design, the synthesis and the physico-chemical characterization of a novel series of alkyl glycosides detergents. The new compounds were able to solubilize membrane proteins from bacterial membranes, insect cells, as well as Madin Darby Canine Kidney (MDCK) cells. In particular, native human adenosine receptor (A2AR) and bacterial transporter BmrA were solubilized efficiently.

A striking thermostability improvement (up to 13°C) was applied to wild type and full length A2AR. Taken together, this novel detergent series shows promising detergent potency for solubilization and stabilization of MPs and, thus, makes a valuable addition to the chemical toolbox available for extracting and handling these important but challenging membrane protein targets.

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