NK1R, Active Human Recombinant GPCR
Full-length human NK1R (neurokinin-1 receptor), a class A GPCR, expressed in HEK293F cells with N-terminal His₁₀- and Twin-Strep-tags. Purified under native conditions via affinity and SEC. Functionally validated using aprepitant in ligand-binding assays.
Biological information
N-terminal 6His and Strep-tagged, recombinant, full length human NK1R.
NK1R is a receptor for the tachykinin neuropeptide substance P (SP). NK1R signalling has been implicated in the regulation of many physiological and pathophysiological functions including pain, inflammation, neuronal survival, regulation of cell movement, salivation, depression, stress responses, emotions, reward, neurogenesis, vigilance, cancer progression, and emesis. NK1R is overexpressed in various cancer types and has been shown to promote tumor cell proliferation, migration, and invasion while inhibiting apoptosis.
This recombinant construct corresponds to the full-length human neurokinin-1 receptor (NK1R), a class A G protein-coupled receptor (GPCR), expressed in HEK293F cells. The protein is purified under native conditions using StrepTactin affinity chromatography followed by size-exclusion chromatography. Expression in mammalian cells ensures appropriate post-translational modifications and membrane integration.
The receptor is solubilized in a detergent mix of DDM and CHS compatible with structural and biochemical studies, including cryo-EM and receptor reconstitution. Its activity has been validated through ligand-binding assays using aprepitant, a selective NK1R antagonist, confirming correct folding and pharmacological relevance. This recombinant NK1R is a robust tool for drug discovery and functional characterization.
Product specifications
Chemical data
N-terminal 6His and Strep-tagged, recombinant, full length human NK1R.
NK1R is a receptor for the tachykinin neuropeptide substance P (SP). NK1R signalling has been implicated in the regulation of many physiological and pathophysiological functions including pain, inflammation, neuronal survival, regulation of cell movement, salivation, depression, stress responses, emotions, reward, neurogenesis, vigilance, cancer progression, and emesis. NK1R is overexpressed in various cancer types and has been shown to promote tumor cell proliferation, migration, and invasion while inhibiting apoptosis.
This recombinant construct corresponds to the full-length human neurokinin-1 receptor (NK1R), a class A G protein-coupled receptor (GPCR), expressed in HEK293F cells. The protein is purified under native conditions using StrepTactin affinity chromatography followed by size-exclusion chromatography. Expression in mammalian cells ensures appropriate post-translational modifications and membrane integration.
The receptor is solubilized in a detergent mix of DDM and CHS compatible with structural and biochemical studies, including cryo-EM and receptor reconstitution. Its activity has been validated through ligand-binding assays using aprepitant, a selective NK1R antagonist, confirming correct folding and pharmacological relevance. This recombinant NK1R is a robust tool for drug discovery and functional characterization.
Product specifications
Lipid Kinase Activity Assay Biological information
N-terminal 6His and Strep-tagged, recombinant, full length human NK1R.
NK1R is a receptor for the tachykinin neuropeptide substance P (SP). NK1R signalling has been implicated in the regulation of many physiological and pathophysiological functions including pain, inflammation, neuronal survival, regulation of cell movement, salivation, depression, stress responses, emotions, reward, neurogenesis, vigilance, cancer progression, and emesis. NK1R is overexpressed in various cancer types and has been shown to promote tumor cell proliferation, migration, and invasion while inhibiting apoptosis.
This recombinant construct corresponds to the full-length human neurokinin-1 receptor (NK1R), a class A G protein-coupled receptor (GPCR), expressed in HEK293F cells. The protein is purified under native conditions using StrepTactin affinity chromatography followed by size-exclusion chromatography. Expression in mammalian cells ensures appropriate post-translational modifications and membrane integration.
The receptor is solubilized in a detergent mix of DDM and CHS compatible with structural and biochemical studies, including cryo-EM and receptor reconstitution. Its activity has been validated through ligand-binding assays using aprepitant, a selective NK1R antagonist, confirming correct folding and pharmacological relevance. This recombinant NK1R is a robust tool for drug discovery and functional characterization.
SPR; NK1R; NKIR; TAC1R; NK-1R
Lipid Kinase Activity Assay Usage
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Lipid Kinase Activity Assay Information
Biological Information
N-terminal 6His and Strep-tagged, recombinant, full length human NK1R.
NK1R is a receptor for the tachykinin neuropeptide substance P (SP). NK1R signalling has been implicated in the regulation of many physiological and pathophysiological functions including pain, inflammation, neuronal survival, regulation of cell movement, salivation, depression, stress responses, emotions, reward, neurogenesis, vigilance, cancer progression, and emesis. NK1R is overexpressed in various cancer types and has been shown to promote tumor cell proliferation, migration, and invasion while inhibiting apoptosis.
This recombinant construct corresponds to the full-length human neurokinin-1 receptor (NK1R), a class A G protein-coupled receptor (GPCR), expressed in HEK293F cells. The protein is purified under native conditions using StrepTactin affinity chromatography followed by size-exclusion chromatography. Expression in mammalian cells ensures appropriate post-translational modifications and membrane integration.
The receptor is solubilized in a detergent mix of DDM and CHS compatible with structural and biochemical studies, including cryo-EM and receptor reconstitution. Its activity has been validated through ligand-binding assays using aprepitant, a selective NK1R antagonist, confirming correct folding and pharmacological relevance. This recombinant NK1R is a robust tool for drug discovery and functional characterization.
Product Specifications
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Biological Information
N-terminal 6His and Strep-tagged, recombinant, full length human NK1R.
NK1R is a receptor for the tachykinin neuropeptide substance P (SP). NK1R signalling has been implicated in the regulation of many physiological and pathophysiological functions including pain, inflammation, neuronal survival, regulation of cell movement, salivation, depression, stress responses, emotions, reward, neurogenesis, vigilance, cancer progression, and emesis. NK1R is overexpressed in various cancer types and has been shown to promote tumor cell proliferation, migration, and invasion while inhibiting apoptosis.
This recombinant construct corresponds to the full-length human neurokinin-1 receptor (NK1R), a class A G protein-coupled receptor (GPCR), expressed in HEK293F cells. The protein is purified under native conditions using StrepTactin affinity chromatography followed by size-exclusion chromatography. Expression in mammalian cells ensures appropriate post-translational modifications and membrane integration.
The receptor is solubilized in a detergent mix of DDM and CHS compatible with structural and biochemical studies, including cryo-EM and receptor reconstitution. Its activity has been validated through ligand-binding assays using aprepitant, a selective NK1R antagonist, confirming correct folding and pharmacological relevance. This recombinant NK1R is a robust tool for drug discovery and functional characterization.
Product Specifications
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.