STK39, active
N-terminal GST-tagged recombinant, human STK39 full length and N-terminal GST-tagged recombinant, human MO25α full length.
No items found.
Biological information
Background
STK39 (Serine/Threonine Kinase 39), also known as SPAK (Ste20-related Proline Alanine-rich Kinase), is a serine/threonine protein kinase that is involved in the regulation of ion transport and cell volume in response to changes in osmolarity. STK39 phosphorylates and activates several ion transporters and channels, including the sodium-potassium-chloride cotransporter (NKCC1) and the potassium chloride cotransporter (KCC2), and it has been suggested to play a role in the regulation of blood pressure and salt-sensitive hypertension. Research has also suggested that STK39 may play a role in the regulation of other cellular processes, including cell proliferation, cell cycle progression, and apoptosis. Additionally, STK39 has been implicated in several diseases, including hypertension, diabetes, and cancer, and efforts to develop small-molecule inhibitors of the kinase are underway as potential therapies for these conditions.
Target class
Kinase
Family
STE
Accession number
NM_013233.1; NM_016289.2
Target Name
STK39, active
Target Alias
Origin
Human
Theori. MW
91 kDa (STK39), 67 kDa (MO25α)
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 96% (combined) by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
STK39 (Serine/Threonine Kinase 39), also known as SPAK (Ste20-related Proline Alanine-rich Kinase), is a serine/threonine protein kinase that is involved in the regulation of ion transport and cell volume in response to changes in osmolarity. STK39 phosphorylates and activates several ion transporters and channels, including the sodium-potassium-chloride cotransporter (NKCC1) and the potassium chloride cotransporter (KCC2), and it has been suggested to play a role in the regulation of blood pressure and salt-sensitive hypertension. Research has also suggested that STK39 may play a role in the regulation of other cellular processes, including cell proliferation, cell cycle progression, and apoptosis. Additionally, STK39 has been implicated in several diseases, including hypertension, diabetes, and cancer, and efforts to develop small-molecule inhibitors of the kinase are underway as potential therapies for these conditions.
Compound name
Kinase
Catalog number
16-049
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
STK39 (Serine/Threonine Kinase 39), also known as SPAK (Ste20-related Proline Alanine-rich Kinase), is a serine/threonine protein kinase that is involved in the regulation of ion transport and cell volume in response to changes in osmolarity. STK39 phosphorylates and activates several ion transporters and channels, including the sodium-potassium-chloride cotransporter (NKCC1) and the potassium chloride cotransporter (KCC2), and it has been suggested to play a role in the regulation of blood pressure and salt-sensitive hypertension. Research has also suggested that STK39 may play a role in the regulation of other cellular processes, including cell proliferation, cell cycle progression, and apoptosis. Additionally, STK39 has been implicated in several diseases, including hypertension, diabetes, and cancer, and efforts to develop small-molecule inhibitors of the kinase are underway as potential therapies for these conditions.
Target class
Kinase
Family
STE
Subfamily
Protein Name
STK39
Protein Alias
Accession Number
NM_013233.1; NM_016289.2
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures