TSSK4, active
N-terminal GST tagged and Cterminal 6His tagged, recombinant, human TSSK4 full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity and size exclusion chromatography.
No items found.
Biological information
Background
TSSK4 (Testis-specific serine/threonine-protein kinase 4) is a serine/threonine protein kinase that is primarily expressed in the testis, where it plays a role in the regulation of spermatogenesis. TSSK4 has been shown to be involved in the regulation of sperm motility and morphology, and alterations in TSSK4 expression and activity have been linked to male infertility. Research has suggested that TSSK4 may also play a role in cancer. TSSK4 has been shown to be overexpressed in several types of cancer, including breast cancer, and high levels of TSSK4 expression have been associated with poor prognosis. Additionally, TSSK4 has been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Target class
Kinase
Family
CMGC
Accession number
AY461663.1
Target Name
TSSK4, active
Target Alias
Origin
Human
Theori. MW
65 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 46% by SDS-PAGE and Coomassie blue staining
Purification method
Immobilized metal affinity and size exclusion chromatography
Sample Buffer
0.45mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
TSSK4 (Testis-specific serine/threonine-protein kinase 4) is a serine/threonine protein kinase that is primarily expressed in the testis, where it plays a role in the regulation of spermatogenesis. TSSK4 has been shown to be involved in the regulation of sperm motility and morphology, and alterations in TSSK4 expression and activity have been linked to male infertility. Research has suggested that TSSK4 may also play a role in cancer. TSSK4 has been shown to be overexpressed in several types of cancer, including breast cancer, and high levels of TSSK4 expression have been associated with poor prognosis. Additionally, TSSK4 has been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Compound name
Kinase
Catalog number
15-017
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
TSSK4 (Testis-specific serine/threonine-protein kinase 4) is a serine/threonine protein kinase that is primarily expressed in the testis, where it plays a role in the regulation of spermatogenesis. TSSK4 has been shown to be involved in the regulation of sperm motility and morphology, and alterations in TSSK4 expression and activity have been linked to male infertility. Research has suggested that TSSK4 may also play a role in cancer. TSSK4 has been shown to be overexpressed in several types of cancer, including breast cancer, and high levels of TSSK4 expression have been associated with poor prognosis. Additionally, TSSK4 has been suggested as a potential therapeutic target in cancer, and efforts to develop small-molecule inhibitors of the kinase are underway.
Target class
Kinase
Family
CMGC
Subfamily
Protein Name
TSSK4
Protein Alias
Accession Number
AY461663.1
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures