SIK3, active
N-terminal GST and C-terminal 6His-tagged, recombinant, human SIK3 amino acids 1-307.
No items found.
Biological information
Background
SIK (Myocardial Snf1-Like Kinase or Msk) was initially identified as a Ser/Thr kinase with homology to yeast Snf1 that is involved in mouse cardiogenesis. The human gene maps to a chromosomal region associated with congenital heart defects in Down's syndrome. Snf1 is a histone kinase (H3, Ser10) that may be involved in transcriptional regulation; a role for SIK in histone modification has not yet been firmly established. SIK3 IS involved in positive regulation of TORC1 signaling, positive regulation of TORC2 signaling, and protein phosphorylation. [provided by Alliance of Genome Resources, Apr 2022]
Target class
Kinase
Family
CAMK
Accession number
BC128510.1
Target Name
SIK3, active
Target Alias
SIK Family Kinase 3; QSK; Salt-Inducible Kinase 3; L19; Serine/Threonine-Protein Kinase SIK3
Origin
Human
Theori. MW
63 kDa
Affinity tag
GST, 6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 76% by SDS-PAGE and Coomassie blue staining
Purification method
Immobilised metal affinity chromatography
Sample Buffer
enzyme in 25 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.05 mM EGTA, 50% (v/v) glycerol, 75 mM imidazole, 0.5 mM benzamidine, 0.1 mM PMSF, 0.05% 2-mercaptoethanol. Frozen solution
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
SIK (Myocardial Snf1-Like Kinase or Msk) was initially identified as a Ser/Thr kinase with homology to yeast Snf1 that is involved in mouse cardiogenesis. The human gene maps to a chromosomal region associated with congenital heart defects in Down's syndrome. Snf1 is a histone kinase (H3, Ser10) that may be involved in transcriptional regulation; a role for SIK in histone modification has not yet been firmly established. SIK3 IS involved in positive regulation of TORC1 signaling, positive regulation of TORC2 signaling, and protein phosphorylation. [provided by Alliance of Genome Resources, Apr 2022]
Compound name
Kinase
Catalog number
15-011
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
SIK (Myocardial Snf1-Like Kinase or Msk) was initially identified as a Ser/Thr kinase with homology to yeast Snf1 that is involved in mouse cardiogenesis. The human gene maps to a chromosomal region associated with congenital heart defects in Down's syndrome. Snf1 is a histone kinase (H3, Ser10) that may be involved in transcriptional regulation; a role for SIK in histone modification has not yet been firmly established. SIK3 IS involved in positive regulation of TORC1 signaling, positive regulation of TORC2 signaling, and protein phosphorylation. [provided by Alliance of Genome Resources, Apr 2022]
Target class
Kinase
Family
CAMK
Subfamily
Protein Name
SIK3
Protein Alias
SIK Family Kinase 3; QSK; Salt-Inducible Kinase 3; L19; Serine/Threonine-Protein Kinase SIK3
Accession Number
BC128510.1
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures