SIK2, active
N-terminal GST-tagged, recombinant, human SIK2 amino acids 1-276 expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.
No items found.
Biological information
Background
SIK2 phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. It inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.
Target class
Kinase
Family
CMGC
Accession number
NM_015191.1
Target Name
SIK2, active
Target Alias
Origin
Human
Theori. MW
59 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 76% by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathion agarose
Sample Buffer
1.98 mg/ml of enzyme in 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
SIK2 phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. It inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.
Compound name
Kinase
Catalog number
15-010
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
SIK2 phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. It inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators.
Target class
Kinase
Family
CMGC
Subfamily
Protein Name
SIK2
Protein Alias
Accession Number
NM_015191.1
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures