Wee1, active
10 µg of active Wee1. N-terminal GST-tagged and C-terminal 6His-tagged recombinant, human Wee1 amino acids 214-end.
No items found.
Biological information
Background
Wee1 is a protein kinase that plays an important role in the regulation of the cell cycle, particularly in the G2 checkpoint. It functions to inhibit the activity of cyclin-dependent kinase 1 (CDK1), a key regulator of the G2/M transition, by phosphorylating it on a specific tyrosine residue. This phosphorylation inactivates CDK1, preventing the cell from entering mitosis until DNA replication and repair processes have been completed. Research has suggested that Wee1 plays a role in several diseases, including cancer and neurodegenerative diseases. In cancer, inhibition of Wee1 has been suggested as a potential therapy to sensitize cancer cells to DNA damage and improve the efficacy of chemotherapy and radiotherapy. Additionally, Wee1 has been implicated in the regulation of neuronal survival and degeneration, making it a potential target for the treatment of neurodegenerative diseases.
Target class
Kinase
Family
CK1
Accession number
NM_003390
Target Name
Wee1, active
Target Alias
WEE1
Origin
Human
Theori. MW
77 kDa
Affinity tag
GST, 6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 86.3% by SDS-PAGE and Coomassie
blue staining.
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Wee1 is a protein kinase that plays an important role in the regulation of the cell cycle, particularly in the G2 checkpoint. It functions to inhibit the activity of cyclin-dependent kinase 1 (CDK1), a key regulator of the G2/M transition, by phosphorylating it on a specific tyrosine residue. This phosphorylation inactivates CDK1, preventing the cell from entering mitosis until DNA replication and repair processes have been completed. Research has suggested that Wee1 plays a role in several diseases, including cancer and neurodegenerative diseases. In cancer, inhibition of Wee1 has been suggested as a potential therapy to sensitize cancer cells to DNA damage and improve the efficacy of chemotherapy and radiotherapy. Additionally, Wee1 has been implicated in the regulation of neuronal survival and degeneration, making it a potential target for the treatment of neurodegenerative diseases.
Compound name
Kinase
Catalog number
14-925
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
Wee1 is a protein kinase that plays an important role in the regulation of the cell cycle, particularly in the G2 checkpoint. It functions to inhibit the activity of cyclin-dependent kinase 1 (CDK1), a key regulator of the G2/M transition, by phosphorylating it on a specific tyrosine residue. This phosphorylation inactivates CDK1, preventing the cell from entering mitosis until DNA replication and repair processes have been completed. Research has suggested that Wee1 plays a role in several diseases, including cancer and neurodegenerative diseases. In cancer, inhibition of Wee1 has been suggested as a potential therapy to sensitize cancer cells to DNA damage and improve the efficacy of chemotherapy and radiotherapy. Additionally, Wee1 has been implicated in the regulation of neuronal survival and degeneration, making it a potential target for the treatment of neurodegenerative diseases.
Target class
Kinase
Family
CK1
Subfamily
Protein Name
WEE1
Protein Alias
WEE1
Accession Number
NM_003390
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures