Tec, activated
N-terminal 6His-tagged recombinant, human Tec amino acids 174-end. Autoactivated by incubating with Mg/ATP, excess ATP and MgAc removed by gel filtration.
No items found.
Biological information
Background
TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. It plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-215'. May also be involved in the regulation of osteoclast differentiation.
GenBank NM_003215. The recombinant protein contains the amino acid substitution F514V with reference to NM_003215. F514V is reported inGenBank CB985022.
Target class
Kinase
Family
TK
Accession number
NM_003215
Target Name
Tec, activated
Target Alias
TEC, PSCTK4
Origin
Human
Theori. MW
57 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 5mM β-glycerophosphate, 1mM Na<sub>3</sub>VO<sub>4</sub>. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. It plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-215'. May also be involved in the regulation of osteoclast differentiation.
GenBank NM_003215. The recombinant protein contains the amino acid substitution F514V with reference to NM_003215. F514V is reported inGenBank CB985022.
Compound name
Kinase
Catalog number
14-801
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
TEC is involved in G protein-coupled receptor- and integrin-mediated signalings in blood platelets. It plays a role in hepatocyte proliferation and liver regeneration and is involved in HGF-induced ERK signaling pathway. TEC regulates also FGF2 unconventional secretion (endoplasmic reticulum (ER)/Golgi-independent mechanism) under various physiological conditions through phosphorylation of FGF2 'Tyr-215'. May also be involved in the regulation of osteoclast differentiation.
GenBank NM_003215. The recombinant protein contains the amino acid substitution F514V with reference to NM_003215. F514V is reported inGenBank CB985022.
Target class
Kinase
Family
TK
Subfamily
Protein Name
Tec
Protein Alias
TEC, PSCTK4
Accession Number
NM_003215
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures