Plk1, active
N-terminal 6His-tagged recombinant, human Plk1.
No items found.
Biological information
Background
Polo-like kinases (PLKs) are a family of serine/threonine kinases related to the polo gene product of Drosophila melanogaster, originally identified from a mitotic mutant that displayed abnormal spindle poles. PLKs play essential roles in several stages of mitosis and cytokinesis with a dynamic pattern of localization to centrosomes, kinetochores and central spindle structures during the cell cycle. PLK1 has been shown to be overexpressed in many human tumours, such as non-small cell lung cancer, squamous cell carcinomas of the head and neck, melanomas, oropharyngeal carcinomas, and ovarian and endometrial carcinomas.
GenBank NM_005030
Target class
Kinase
Family
Other
Accession number
NM_005030
Target Name
Plk1, active
Target Alias
PLK, PLK1, STPK13
Origin
Human
Theori. MW
69.3 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Polo-like kinases (PLKs) are a family of serine/threonine kinases related to the polo gene product of Drosophila melanogaster, originally identified from a mitotic mutant that displayed abnormal spindle poles. PLKs play essential roles in several stages of mitosis and cytokinesis with a dynamic pattern of localization to centrosomes, kinetochores and central spindle structures during the cell cycle. PLK1 has been shown to be overexpressed in many human tumours, such as non-small cell lung cancer, squamous cell carcinomas of the head and neck, melanomas, oropharyngeal carcinomas, and ovarian and endometrial carcinomas.
GenBank NM_005030
Compound name
Kinase
Catalog number
14-777
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
Polo-like kinases (PLKs) are a family of serine/threonine kinases related to the polo gene product of Drosophila melanogaster, originally identified from a mitotic mutant that displayed abnormal spindle poles. PLKs play essential roles in several stages of mitosis and cytokinesis with a dynamic pattern of localization to centrosomes, kinetochores and central spindle structures during the cell cycle. PLK1 has been shown to be overexpressed in many human tumours, such as non-small cell lung cancer, squamous cell carcinomas of the head and neck, melanomas, oropharyngeal carcinomas, and ovarian and endometrial carcinomas.
GenBank NM_005030
Target class
Kinase
Family
Other
Subfamily
Protein Name
Plk1
Protein Alias
PLK, PLK1, STPK13
Accession Number
NM_005030
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures