mTOR (1362-end), active
N-terminal FLAG-tagged recombinant, human mTOR amino acids 1362-end.
No items found.
Biological information
Background
mTOR (Mechanistic Target Of Rapamycin Kinase) belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections.
GenBank NM_004958
Target class
Kinase
Family
Lipid
Accession number
NM_004958.2
Target Name
mTOR (1362-end), active
Target Alias
FRAP1, RAPT1, MTOR, FRAP, mTOR, FLJ44809, FRAP2, RAFT1
Origin
Human
Theori. MW
137.3 kDa
Affinity tag
FLAG
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 55% by SDS-PAGE and Coomassie blue staining.
Purification method
FLAG™ agarose
Sample Buffer
Protein in 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 300µg/ml FLAGTM peptide, 20% v/v glycerol
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
mTOR (Mechanistic Target Of Rapamycin Kinase) belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections.
GenBank NM_004958
Compound name
Kinase
Catalog number
14-770
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-770
Datasheet