Tie2 (Y897S), active
N-terminal 6His-tagged recombinant, human Tie2 amino acids 771-end. The Y897S substitution is a gain-of-function mutation associated with hereditary venous malformations (Calvert et al., (1999), Hum. Mol. Genet. 8, 1279-1289).
No items found.
Biological information
Background
The Tie family of receptor tyrosine kinases comprises two members, Tie1 and Tie2 (Angiopoietin 1 receptor, Tyrosine-protein kinase receptor Tie-2, Tyrosine-protein kinase receptor TEK, P140 TEK, Tunica interna endothelial cell kinase, CD202b antigen, EC 2.7.1.112 ). These receptors are expressed predominantly in endothelial cells and are essential for vessel formation where they are required for the later stages of angiogenesis and vessel maintenance. Tie2 has roles in control of microvessel sprouting, integrity, and maturation. Angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) have been identified as ligands of Tie2 receptor, which may regulate endothelial cell proliferation, differentiation and guide the proper patterning of endothelial cells during blood vessel formation. Inhibitors of Tie2 may inhibit vascular healing and tumor angiogenesis.
Target class
Kinase
Family
TK
Accession number
NM_000459
Target Name
Tie2 (Y897S), active
Target Alias
TEK, TIE2
Origin
Human
Theori. MW
42 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The Tie family of receptor tyrosine kinases comprises two members, Tie1 and Tie2 (Angiopoietin 1 receptor, Tyrosine-protein kinase receptor Tie-2, Tyrosine-protein kinase receptor TEK, P140 TEK, Tunica interna endothelial cell kinase, CD202b antigen, EC 2.7.1.112 ). These receptors are expressed predominantly in endothelial cells and are essential for vessel formation where they are required for the later stages of angiogenesis and vessel maintenance. Tie2 has roles in control of microvessel sprouting, integrity, and maturation. Angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) have been identified as ligands of Tie2 receptor, which may regulate endothelial cell proliferation, differentiation and guide the proper patterning of endothelial cells during blood vessel formation. Inhibitors of Tie2 may inhibit vascular healing and tumor angiogenesis.
Compound name
Kinase
Catalog number
14-764
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
The Tie family of receptor tyrosine kinases comprises two members, Tie1 and Tie2 (Angiopoietin 1 receptor, Tyrosine-protein kinase receptor Tie-2, Tyrosine-protein kinase receptor TEK, P140 TEK, Tunica interna endothelial cell kinase, CD202b antigen, EC 2.7.1.112 ). These receptors are expressed predominantly in endothelial cells and are essential for vessel formation where they are required for the later stages of angiogenesis and vessel maintenance. Tie2 has roles in control of microvessel sprouting, integrity, and maturation. Angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) have been identified as ligands of Tie2 receptor, which may regulate endothelial cell proliferation, differentiation and guide the proper patterning of endothelial cells during blood vessel formation. Inhibitors of Tie2 may inhibit vascular healing and tumor angiogenesis.
Target class
Kinase
Family
TK
Subfamily
Protein Name
Tie2
Protein Alias
TEK, TIE2
Accession Number
NM_000459
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures