Tie2 (R849W), active
10 µg of active Tie 2 (R849W). N-terminal 6His-tagged recombinant, human Tie2 amino acids 771-end containing the R849W substitution.
No items found.
Biological information
Background
N-terminal 6His-tagged recombinant, human Tie2 amino acids 771-end containing the R849W substitution. The R849W mutation has been reported in dominantly inherited venous malformations. Expression studies in insect and mammalian cells demonstrates that Tie2 R849W has constitutively elevated tyrosine kinase activity compared with wild type. (Morris P.N., et al., Biochem.Biophys.Res.Commun.(2006);346:335-338 and Vikkula M, et al., Cell(1996);87:1181-1190).
Target class
Kinase
Family
TK
Accession number
NM_000459
Target Name
Tie2 (R849W), active
Target Alias
TEK, TIE2
Origin
Human
Theori. MW
42 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
N-terminal 6His-tagged recombinant, human Tie2 amino acids 771-end containing the R849W substitution. The R849W mutation has been reported in dominantly inherited venous malformations. Expression studies in insect and mammalian cells demonstrates that Tie2 R849W has constitutively elevated tyrosine kinase activity compared with wild type. (Morris P.N., et al., Biochem.Biophys.Res.Commun.(2006);346:335-338 and Vikkula M, et al., Cell(1996);87:1181-1190).
Compound name
Kinase
Catalog number
14-763
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
N-terminal 6His-tagged recombinant, human Tie2 amino acids 771-end containing the R849W substitution. The R849W mutation has been reported in dominantly inherited venous malformations. Expression studies in insect and mammalian cells demonstrates that Tie2 R849W has constitutively elevated tyrosine kinase activity compared with wild type. (Morris P.N., et al., Biochem.Biophys.Res.Commun.(2006);346:335-338 and Vikkula M, et al., Cell(1996);87:1181-1190).
Target class
Kinase
Family
TK
Subfamily
Protein Name
Tie2
Protein Alias
TEK, TIE2
Accession Number
NM_000459
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures