c-Kit (V654A), active
10 µg of active c-Kit. N-terminal GST-tagged recombinant, human c-Kit amino acids 544 - end containing the V654A mutation.
No items found.
Biological information
Background
c-Kit (V654A) is an acquired resistance mutation which has been found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance and rapid progession of GISTs. (Chen LL et al., Cancer Research, (2004); 64:5913-5919).
Target class
Kinase
Family
TK
Accession number
NM_000222.2; NM_001093772.1
Target Name
c-Kit (V654A), active
Target Alias
KIT, C-Kit, PBT, CD117, SCFR, c-kit
Origin
Human
Theori. MW
76.7 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 99% by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
c-Kit (V654A) is an acquired resistance mutation which has been found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance and rapid progession of GISTs. (Chen LL et al., Cancer Research, (2004); 64:5913-5919).
Compound name
Kinase
Catalog number
14-733
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
c-Kit (V654A) is an acquired resistance mutation which has been found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance and rapid progession of GISTs. (Chen LL et al., Cancer Research, (2004); 64:5913-5919).
Target class
Kinase
Family
TK
Subfamily
Protein Name
c-Kit
Protein Alias
KIT, C-Kit, PBT, CD117, SCFR, c-kit
Accession Number
NM_000222.2; NM_001093772.1
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures