Custom Recombinant Protein Production Services
Having trouble sourcing recombinant proteins that are truly functional and correctly folded?
Eurofins CALIXAR is a Contract Research Organization (CRO) specialized in the expression, purification, and qualification of the most complex membrane and soluble proteins, from GPCRs to kinases. We deliver fully functional, application-ready proteins with scientific rigor, native conformation, and high compatibility with structural biology, drug discovery, and antibody development workflows. From construct design to large-scale production, we ensure functional integrity, biological activity, and seamless integration into your downstream pipeline.
From Misfolded to Functional: Solving Complex Expression Challenges
Expressing complex proteins, such as membrane-bound or multi-domain targets, frequently leads to low yield, aggregation, or loss of activity. These issues can compromise downstream workflows, delay timelines, and reduce confidence in screening or structural results.
At Eurofins CALIXAR, we address these challenges by designing target-specific production strategies that ensure:
- Native conformation and folding
- Full-length structure preservation
- Verified biological activity and stability
- Compatibility with your final application , including high-throughput screening (HTS), immunization protocols, structural studies using cryo-electron microscopy (cryo-EM), and more.
With over 20 years of experience, we support pharmaceutical, biotech and academic teams advance discovery with reliable, reproducible protein tools — right from the start.
🎯 Struggling with difficult targets? Let’s design your protein production strategy.
A Fully Integrated CRO for Functional Membrane and Soluble Proteins
Eurofins CALIXAR is a specialized CRO with end-to-end capabilities in protein production. Every step is managed internally, from construct design to purification and quality control, using our proprietary high-quality process developed for functional performance and reproducibility.
Our workflows are engineered to support:
- Drug discovery campaigns based on high-throughput screening, surface plasmon resonance (SPR), microscale thermophoresis (MST), or DNA-encoded libraries.
- Structural biology efforts such as cryo-EM, X-ray crystallography, and structure-based drug design (SBDD).
- Antibody development programs using immunization, phage display, yeast display,
nanobody discovery… - Diagnostic assays and mechanistic studies (ligand binding, enzyme activity, signal transduction, inhibition/activation studies…).
Each production strategy is customized to align with the technical and functional requirements of your application.
This level of scientific control and application focus has made Eurofins CALIXAR a trusted CRO partner for pharmaceutical, biotech, and academic institutions worldwide.
📌 Looking for more than just protein quantity? Let’s define your success criteria.
750+
20+
100%
92%
At Eurofins CALIXAR, we begin each project with an in-depth scientific assessment:
- Sequence and domain architecture
- Expression system compatibility
- Functional, structural, or immunogenic requirements
- Intended application
This groundwork enables the design of production strategies tailored to your target — not based on generic protocols, but optimized for native folding, biological activity, and application-specific format compatibility.
We manage the entire workflow in-house, from construct design and expression to solubilization, stabilization, purification, characterization, large-scale production, and final protein identification , ensuring both scientific consistency and the flexibility to adapt to your internal workflows or project stage.
Each of these steps is executed using our high-quality proprietary process, designed to maximize reproducibility, functional relevance, and downstream compatibility.
Clients can rely on the complete process or select specific modules depending on their needs.
▶️ Explore our workflow
From Sequence to Functional Protein: Integrated and Customizable
01
Protein Construct Design
Protein Construct Design
Protein Construct Design
Protein constructs with custom tags/fusion align with your project goals.
02
Protein Expression
Protein Expression
Protein Expression
Cutting-edge technology ensures high-yield, optimal protein performance.
03
Protein Solubilization & Stabilization
Protein Solubilization & Stabilization
Protein Solubilization & Stabilization
Expert techniques enhance solubility and stability using proprietary reagents.
04
Protein Purification
Protein Purification
Protein Purification
State-of-the-art methods isolate proteins with efficiency and quality.
05
Protein Characterization
Protein Characterization
Protein Characterization
Comprehensive analysis provides insights into structural and functional attributes.
06
Large-Scale Protein Production
Large-Scale Protein Production
Large-Scale Protein Production
Trust us to scale up your protein target with quality and quantity in mind.
07
Protein Identification
Protein Identification
Protein Identification
Precision protein identification for unrivaled structural and functional insights.
Membrane and Soluble Protein Expertise, Built for Scientific Performance
Eurofins CALIXAR excels in expressing protein targets that are often considered too complex or risky by conventional providers, including low-expression constructs, hydrophobic sequences, and structurally intricate, multi-domain proteins.
For both membrane and soluble targets, we tailor every step of the production process to preserve native structure, biological activity, and application compatibility.
- Membrane Proteins
We produce GPCRs, ion channels, transporters, and other transmembrane proteins using formats such as detergents, nanodiscs, and proteoliposomes. These systems are selected and validated to ensure proper folding, functional orientation, and downstream performance — from cryo-EM to ligand binding and transport studies. - Soluble Proteins
Kinases, helicases, phosphatases, epigenetic regulators, and other intracellular enzymes are produced in full-length, active forms that retain their structural domains and catalytic activity. Delivered in stable, assay-ready formats, they integrate seamlessly into screening, immunization, or mechanistic workflows.
Each protein is delivered as a validated, ready-to-use reagent, supported by comprehensive
documentation and QC aligned with your experimental needs.
documentation and QC aligned with your experimental needs.
Need some help from our expert to assess the feasibility of your project?
Application-Driven Formats for Functional Membrane Proteins
Membrane proteins require formats that preserve structural integrity under biochemical constraints. We offer empirically validated options, selected based on the target’s profile and application needs.
These applications include techniques such as high-throughput screening (HTS), cryo-electron microscopy (cryo-EM), surface plasmon resonance (SPR), phage and yeast display, and immunization — each requiring format-specific optimization.
| Membrane protein formats | ||||
|---|---|---|---|---|
| Protein-based applications | Detergents | Amphipols | Nanodiscs | Proteoliposomes |
| Small Molecule Screening | ||||
| MST / Spectral shift | ✓ | ✓ | ✓ | |
| SPR | ✓ | ✓ | ✓ | |
| AS-MS | ✓ | ✓ | ||
| DEL | ✓ | |||
| Antibody Discovery | ||||
| Immunization | ✓ | ✓ | ||
| Phage display | ✓ | ✓ | ||
| Yeast display | ✓ | ✓ | ||
| Structure Determination | ||||
| Cryo-EM | ✓ | ✓ | ✓ | |
| X-ray | ✓ | |||
| Bioassay Development | ||||
| Enzymatic assay | ✓ | ✓ | ✓ | ✓ |
| Transport assay | ✓ | ✓ | ✓ | ✓ |
| ELISA | ✓ | ✓ | ✓ | ✓ |
| Vaccine Discovery | ||||
| Subunit | ✓ | |||
🧠 Not sure which format suits your protein best?
We help define the optimal strategy based on your application:
Deliverables & Scientific Collaboration, More Than Just a Protein
Eurofins CALIXAR delivers not just proteins, but high-performance reagents designed to integrate seamlessly into your discovery workflows. Each project is backed by precise documentation, validated data, and ongoing scientific interaction.
- What You Receive:
- Custom Recombinant Protein:
- Delivered frozen in detergents, amphipols, nanodiscs, or proteoliposomes
- Formulated based on target-specific biophysical and application requirements
- Project Documentation:
- Technical report including construct design, purification workflow, and QC results
- Buffer composition, storage conditions, and usage recommendations
- Custom Recombinant Protein:
- How We Work With You:
- Scientific Leadership:
- Each project is led by a PhD-level protein expert
- Clear communication:
- Kick-off meeting to define technical goals and success criteria
- Weekly updates with Gantt timeline and milestone tracking
- End-of-stage meetings with results review and recommendations
- Real-time access:
- On-demand availability of your scientific coordinator
- Direct scientist-to-scientist interactions for critical decisions and alignment
- Scientific Leadership:
With Eurofins CALIXAR, you gain more than a deliverable — you engage a scientific partner committed to functional success. Let’s Build Your Functional Proteins Together.
Expression of fully native proteins
Identification of relevant targets
Target functional isolation and stabilization
Active custom proteins
Structure-based drug discovery
Immunoassays
Vaccine development
Functional antigen discovery
Conformational antibody development
Expression of fully native proteins
Identification of relevant targets
Target functional isolation and stabilization
Active custom proteins
Structure-based drug discovery
Immunoassays
Vaccine development
Functional antigen discovery
Conformational antibody development
Secure and boost
your discovery programs
Starting from native material or recombinant systems, we succeed with all types of proteins: Kinases, Phosphatases, Ubiquitins, Epigenetic Proteins, GPCRs, Ion Channels, Transporters, Receptors and Viral Proteins.
Eurofins CALIXAR’s approach allows to preserve the original structure and function of recombinant proteins (Kinases, Phosphatases, Ubiquitin, Epigenetic Proteins, GPCRs, Ion Channels, Transporters, Receptors, Anchors and Viral Proteins) providing solutions for pharmaceutical industries, biotechnology companies and academic teams to develop conformational antibodies, formulate new vaccines, carry out Structure Based Drug Discovery and/or HTS assays.