SIK, active
N-terminal 6His-tagged recombinant, human SIK amino acids 1-281.
No items found.
Biological information
Background
SIK plays a transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. This protein has a potential role in G2/M cell cycle regulation, and inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3 (By similarity).
Target class
Kinase
Family
CAMK
Accession number
NM_173354.3
Target Name
SIK, active
Target Alias
SNF1LK, MSK, msk, SIK, SIK1
Origin
Human
Theori. MW
36.2 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 52% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5,
300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose,
1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
SIK plays a transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. This protein has a potential role in G2/M cell cycle regulation, and inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3 (By similarity).
Compound name
Kinase
Catalog number
14-652
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
SIK plays a transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. This protein has a potential role in G2/M cell cycle regulation, and inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3 (By similarity).
Target class
Kinase
Family
CAMK
Subfamily
Protein Name
SIK
Protein Alias
SNF1LK, MSK, msk, SIK, SIK1
Accession Number
NM_173354.3
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures