PKG1β, active
N-terminal 6His-tagged recombinant, full length PKG1β.
No items found.
Biological information
Background
In smooth muscle cells, one of the major receptors for cGMP is the serine/threonine protein kinase, cGMP-dependent protein kinase 1, beta isozyme (PRKG1B). PRKG1 has been shown to catalyze the phosphorylation of a number of physiologically relevant proteins whose function it is to regulate the contractile activity of the smooth muscle cell. Two main types of PRKG are present in eukaryotic cells. Type I is widely distributed and is isolated from soluble extracts of tissues, such as cardiac and smooth muscle, as well as lymphoid cells. Type II is a particulate form of the enzyme and has a limited tissue distribution.
GenBank NM_006258
Target class
Kinase
Family
AGC
Accession number
NM_001098512.1; NM_006258.2
Target Name
PKG1β, active
Target Alias
PRKG1, cGKI-beta, FLJ36117, PGK, PRKGR1A, cGKI-alpha, cGKI, MGC71944, OTTHUMP00000061216, CGKI, PRKG1B, cGKI-BETA, PRKGR1B, DKFZp686K042
Origin
Human
Theori. MW
81.6 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
In smooth muscle cells, one of the major receptors for cGMP is the serine/threonine protein kinase, cGMP-dependent protein kinase 1, beta isozyme (PRKG1B). PRKG1 has been shown to catalyze the phosphorylation of a number of physiologically relevant proteins whose function it is to regulate the contractile activity of the smooth muscle cell. Two main types of PRKG are present in eukaryotic cells. Type I is widely distributed and is isolated from soluble extracts of tissues, such as cardiac and smooth muscle, as well as lymphoid cells. Type II is a particulate form of the enzyme and has a limited tissue distribution.
GenBank NM_006258
Compound name
Kinase
Catalog number
14-650
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
In smooth muscle cells, one of the major receptors for cGMP is the serine/threonine protein kinase, cGMP-dependent protein kinase 1, beta isozyme (PRKG1B). PRKG1 has been shown to catalyze the phosphorylation of a number of physiologically relevant proteins whose function it is to regulate the contractile activity of the smooth muscle cell. Two main types of PRKG are present in eukaryotic cells. Type I is widely distributed and is isolated from soluble extracts of tissues, such as cardiac and smooth muscle, as well as lymphoid cells. Type II is a particulate form of the enzyme and has a limited tissue distribution.
GenBank NM_006258
Target class
Kinase
Family
AGC
Subfamily
Protein Name
PKG1β
Protein Alias
PRKG1, cGKI-beta, FLJ36117, PGK, PRKGR1A, cGKI-alpha, cGKI, MGC71944, OTTHUMP00000061216, CGKI, PRKG1B, cGKI-BETA, PRKGR1B, DKFZp686K042
Accession Number
NM_001098512.1; NM_006258.2
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures