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ZIPK, active

N-terminal GST-tagged recombinant, human ZIPK amino acids 1-290.

Biological information

Background

ZIP (or DAPK3 : Death-associated protein kinase) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. It has been shown to have a potential role in regulating VSMC contraction and induces morphological changes in apoptosis when overexpressed in mammalian cells.

Target class
Kinase
Family
CAMK
Accession number
NM_001348.1
Target Name
ZIPK, active
Target Alias
DAPK3, FLJ36473, ZIP-kinase, ZIPK, Dlk, ZIP
Origin
Human
Theori. MW
64.3 kDa
Affinity tag
GST

Product specifications

Expression system
Expressed in E. coli
Purity
Purity 56.9% by SDS-PAGE and Coomassie blue staining.
Purification method
Glutathione agarose affinity chromatography
Sample Buffer
"Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution."
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only

Chemical data

Background

ZIP (or DAPK3 : Death-associated protein kinase) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. It has been shown to have a potential role in regulating VSMC contraction and induces morphological changes in apoptosis when overexpressed in mammalian cells.

Compound name
Kinase
Catalog number
14-608
Molecular formula
CAS
MW
Ka
Percent composition

Product specifications

Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure

Lipid Kinase Activity Assay Biological information

Background

ZIP (or DAPK3 : Death-associated protein kinase) is a calcium/calmodulin-dependent kinase that phosphorylates serine/threonine residues on proteins. It has been shown to have a potential role in regulating VSMC contraction and induces morphological changes in apoptosis when overexpressed in mammalian cells.

Target class
Kinase
Family
CAMK
Subfamily
Protein Name
ZIPK
Protein Alias
DAPK3, FLJ36473, ZIP-kinase, ZIPK, Dlk, ZIP
Accession Number
NM_001348.1
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human

Lipid Kinase Activity Assay Usage

Product Type
Application
Storage Conditions
6 months at -70°C
Usage disclaimer

Lipid Kinase Activity Assay Information

Assay Type
Assay Measures
Datasheets
User Manual
File N°2
€ 389,00 EUR
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