SGK1 (δ1-59, S422D), active
N-truncated (missing N-terminal 60 amino acids), 6His-tagged recombinant, human SGK1 fusion protein, containing a S422D mutation.
No items found.
Biological information
Background
The serum/glucocorticoid regulated kinases (SGK1,2,3) are serine/threonine kinases that belong to the AGC (protein kinase A; protein kinase G and protein kinase C) subfamily that includes cAMP and cGMP dependent kinases, PKB and PKC. SGK1 (SGK) is predominately expressed in kidney, liver and pancreas. The catalytic domain of serum/glucocorticoid regulated protein kinase is most similar to PKB and activated via phosphorlation mechanism when cells are stimulated with insulin, IGF1 or serum, or exposed to oxidative stress.
GenBank AF153609. The protein is S422D with respect to this accession number.
Target class
Kinase
Family
AGC
Accession number
NM_005627.2
Target Name
SGK1 (δ1-59, S422D), active
Target Alias
SGK, SGK1
Origin
Human
Theori. MW
48 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
The serum/glucocorticoid regulated kinases (SGK1,2,3) are serine/threonine kinases that belong to the AGC (protein kinase A; protein kinase G and protein kinase C) subfamily that includes cAMP and cGMP dependent kinases, PKB and PKC. SGK1 (SGK) is predominately expressed in kidney, liver and pancreas. The catalytic domain of serum/glucocorticoid regulated protein kinase is most similar to PKB and activated via phosphorlation mechanism when cells are stimulated with insulin, IGF1 or serum, or exposed to oxidative stress.
GenBank AF153609. The protein is S422D with respect to this accession number.
Compound name
Kinase
Catalog number
14-331
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Lipid Kinase Activity Assay Biological information
Background
The serum/glucocorticoid regulated kinases (SGK1,2,3) are serine/threonine kinases that belong to the AGC (protein kinase A; protein kinase G and protein kinase C) subfamily that includes cAMP and cGMP dependent kinases, PKB and PKC. SGK1 (SGK) is predominately expressed in kidney, liver and pancreas. The catalytic domain of serum/glucocorticoid regulated protein kinase is most similar to PKB and activated via phosphorlation mechanism when cells are stimulated with insulin, IGF1 or serum, or exposed to oxidative stress.
GenBank AF153609. The protein is S422D with respect to this accession number.
Target class
Kinase
Family
AGC
Subfamily
Protein Name
SGK1
Protein Alias
SGK, SGK1
Accession Number
NM_005627.2
UniProt Number
Gene Name
Gene ID
Gene Aliases
Target Species
Human
Lipid Kinase Activity Assay Usage
Product Type
Application
Storage Conditions
1 year at -70°C
Usage disclaimer
Lipid Kinase Activity Assay Information
Assay Type
Assay Measures