MKK3, active
10 µg of active MKK3. N-terminal GST-tagged, recombinant, full length, human MKK3.
Biological information
Background
MKK3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. MKK3 is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of MKK3 is involved in the pathogenesis of Yersina pseudotuberculosis [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Ser/Thr Kinase; MAPK; STE7 Family
Accession number
NM_002756.4
Target Name
MKK3, active
Target Alias
Mitogen-Activated Protein Kinase Kinase 3MAPK/ERK Kinase 3MAP Kinase Kinase 3Dual Specificity Mitogen-Activated Protein Kinase KinaseStress-Activated Protein Kinase Kinase 2Dual Specificity Mitogen Activated Protein Kinase Kinase 3SAPK Kinase 2EC 2.7.12.2
Origin
Human
Theori. MW
63 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
85% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified using glutathione agarose.
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
MKK3 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. MKK3 is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of MKK3 is involved in the pathogenesis of Yersina pseudotuberculosis [provided by RefSeq, Jul 2008]
Compound name
Kinase
Catalog number
16-037
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-037
Datasheet