MEKK3, active
10 µg of active MEKK3. N-terminal GST-tagged, recombinant, human MEKK3 full length.
Biological information
Background
MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. [provided by RefSeq, Jul 2008]
Target class
Kinase
Family
Ser/Thr Kinase
Accession number
NM_002401.3
Target Name
MEKK3, active
Target Alias
Mitogen-Activated Protein Kinase Kinase Kinase 3MAPK/ERK Kinase Kinase 3MAP/ERK Kinase Kinase 3MEK Kinase 3EC 2.7.11.25
Origin
Human
Theori. MW
97.7 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 52% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified using glutathione agarose.
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C.
Usage disclaimer
For Research Only
Chemical data
Background
MEKK3 is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. [provided by RefSeq, Jul 2008]
Compound name
Kinase
Catalog number
16-015
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
16-015
Datasheet