MAP4K4, active
N-terminal GST tagged and C-terminal 6His tagged, recombinant, human MAP4K4 amino acids 1-328, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.
Biological information
Background
MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Target class
Kinase
Family
CMGC
Accession number
NM_004834.4
Target Name
MAP4K4, active
Target Alias
Origin
Human
Theori. MW
66 kDa
Affinity tag
GST
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 98% by SDS-PAGE and Coomassie blue staining.
Purification method
Immobilized metal affinity chromatography
Sample Buffer
3.20 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
MAP4K4 is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
Compound name
Kinase
Catalog number
15-012
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
15-012
Datasheet