NEK9, active
10 µg of active NEK9. N-terminal 6His-tagged recombinant, human NEK9 amino acids 1-324.
No items found.
Biological information
Background
NEK9 (NIMA-related kinase 9) is a serine/threonine protein kinase that belongs to the NEK (never in mitosis gene A-related kinase) family of kinases. It is involved in a variety of cellular processes, including regulation of the cell cycle, mitosis, and the DNA damage response. NEK9 is expressed in a wide range of tissues and cell types, and is regulated by various post-translational modifications, including phosphorylation and ubiquitination. Research has suggested that NEK9 plays a role in several diseases, including cancer and ciliopathies. In cancer, NEK9 has been shown to promote cell proliferation and survival, and inhibition of NEK9 has been suggested as a potential cancer therapy. In ciliopathies, NEK9 has been implicated in the regulation of cilia length and number, and mutations in NEK9 have been associated with ciliopathy-related disorders such as Joubert syndrome.
Target class
Kinase
Family
Other
Accession number
NM_033116
Target Name
NEK9, active
Target Alias
NEK9
Origin
Human
Theori. MW
41 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 84.8% by SDS-PAGE and Coomassie blue staining.
Purification method
Purified
using immobilized metal affinity
chromatography.
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
NEK9 (NIMA-related kinase 9) is a serine/threonine protein kinase that belongs to the NEK (never in mitosis gene A-related kinase) family of kinases. It is involved in a variety of cellular processes, including regulation of the cell cycle, mitosis, and the DNA damage response. NEK9 is expressed in a wide range of tissues and cell types, and is regulated by various post-translational modifications, including phosphorylation and ubiquitination. Research has suggested that NEK9 plays a role in several diseases, including cancer and ciliopathies. In cancer, NEK9 has been shown to promote cell proliferation and survival, and inhibition of NEK9 has been suggested as a potential cancer therapy. In ciliopathies, NEK9 has been implicated in the regulation of cilia length and number, and mutations in NEK9 have been associated with ciliopathy-related disorders such as Joubert syndrome.
Compound name
Kinase
Catalog number
14-936
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-936
Datasheet