PI3-Kinase (p110α(E542K)/p85α) murine
10 µg of murine PI3 Kinase (p110α (E542K)/p85α). Complex of N-terminal 6His-tagged recombinant, murine p110α full length, containing the mutation E542K, and untagged, recombinant, murine p85α full length.
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Biological information
Background
Complex of N-terminal 6His-tagged recombinant, murine p110α full length, containing the mutation E542K, and untagged, recombinant, murine p85α full length. The E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vitro studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation. (Kang S. et al., PNAS, (2005);102: 802-807 and Zhao J.J. et al., PNAS, (2005);102:18443-18448).
Target class
Kinase
Family
PI3K/PI4K
Accession number
BC089038
Target Name
PI3-Kinase (p110α(E542K)/p85α) murine
Target Alias
PIK3CA, PI3K, PK3CA
Origin
Mouse
Theori. MW
129 kDa (p110α E542K), 84 kDa (p85α)
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Complex of N-terminal 6His-tagged recombinant, murine p110α full length, containing the mutation E542K, and untagged, recombinant, murine p85α full length. The E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vitro studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation. (Kang S. et al., PNAS, (2005);102: 802-807 and Zhao J.J. et al., PNAS, (2005);102:18443-18448).
Compound name
Kinase
Catalog number
14-791
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-791
Datasheet