PI3 Kinase (p110α(E545K)/p85α), murine
10 µg of murine PI3 Kinase (p110α(E545K)/p85α). Complex of N-terminal 6His-tagged recombinant, murine full length p110α containing the mutation E545K and untagged, recombinant, full length murine p85α.
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Biological information
Background
Complex of N-terminal 6His-tagged recombinant, murine full length p110α containing the mutation E545K and untagged, recombinant, full length murine p85α. The E545K substitution is associated with a number of human cancers. This residue is conserved between the human and murine sequences of p110α. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.
Target class
Kinase
Family
PI3K/PI4K
Accession number
NM_006218.2
Target Name
PI3 Kinase (p110α(E545K)/p85α), murine
Target Alias
PIK3CA, PI3K, MGC142161, p110-alpha, MGC142163
Origin
Mouse
Theori. MW
129 kDa (p110α Ε545Κ), 83.6 kDa (p85α)
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
Complex of N-terminal 6His-tagged recombinant, murine full length p110α containing the mutation E545K and untagged, recombinant, full length murine p85α. The E545K substitution is associated with a number of human cancers. This residue is conserved between the human and murine sequences of p110α. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.
Compound name
Kinase
Catalog number
14-781
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-781
Datasheet