PASK, active
6His-tagged recombinant, human full length PASK amino acids 995–end.
No items found.
Biological information
Background
PASK kinase is a serine/threonine-protein kinase is involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. It probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.
Target class
Kinase
Family
CAMK
Accession number
NM_015148
Target Name
PASK, active
Target Alias
Origin
Human
Theori. MW
41.5 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 60.9% by SDS-PAGE and Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5,
300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM
benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen
solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
1 year at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
PASK kinase is a serine/threonine-protein kinase is involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. It probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.
Compound name
Kinase
Catalog number
14-701
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-701
Datasheet