NEK11, active
6His-tagged recombinant, human full length NEK11.
No items found.
Biological information
Background
NEK11 is a protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.
Target class
Kinase
Family
Other
Accession number
NM_024800.3; NM_145910.2
Target Name
NEK11, active
Target Alias
NEK11, FLJ23495
Origin
Human
Theori. MW
59.3 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 77% by SDSPAGE and Coomassie blue staining,
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5,
300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM
benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen
solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
NEK11 is a protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation.
Compound name
Kinase
Catalog number
14-700
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-700
Datasheet