NEK3, active
N-terminal 6His-tagged recombinant, human full length NEK3.
No items found.
Biological information
Background
NEK3 (NIMA-related kinase 3) is a serine/threonine protein kinase that belongs to the NIMA (Never in Mitosis A)-related kinase family. It is involved in a variety of cellular processes, including cell cycle regulation, mitosis, apoptosis, and cytoskeletal organization. NEK3 has a conserved kinase domain, a C-terminal regulatory domain, and a nuclear localization signal. It is expressed in a wide range of tissues and cell types and is regulated by various post-translational modifications, including phosphorylation, acetylation, and ubiquitination. Recent studies have suggested that NEK3 plays a critical role in promoting tumor growth and survival, making it a potential target for cancer therapy. Additionally, NEK3 has been implicated in a number of other diseases, including heart disease and Alzheimer's disease.
Target class
Kinase
Family
Other
Accession number
NM_002498.2; NM_152720.1
Target Name
NEK3, active
Target Alias
NEK3, HSPK36, MGC29949
Origin
Human
Theori. MW
62.2 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Purity 84% by SDS-PAGE and
Coomassie blue staining.
Purification method
Ni2+/NTA-agarose
Sample Buffer
Protein in 50mM Tris/HCl pH7.5,
300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM
benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen
solution.
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
NEK3 (NIMA-related kinase 3) is a serine/threonine protein kinase that belongs to the NIMA (Never in Mitosis A)-related kinase family. It is involved in a variety of cellular processes, including cell cycle regulation, mitosis, apoptosis, and cytoskeletal organization. NEK3 has a conserved kinase domain, a C-terminal regulatory domain, and a nuclear localization signal. It is expressed in a wide range of tissues and cell types and is regulated by various post-translational modifications, including phosphorylation, acetylation, and ubiquitination. Recent studies have suggested that NEK3 plays a critical role in promoting tumor growth and survival, making it a potential target for cancer therapy. Additionally, NEK3 has been implicated in a number of other diseases, including heart disease and Alzheimer's disease.
Compound name
Kinase
Catalog number
14-694
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-694
Datasheet