MARK1, active
N-terminal 6His-tagged recombinant, human MARK1 amino acids 2-end.
No items found.
Biological information
Background
MARK (MAP/microtubule affinity-regulating kinase) phosphorylates the microtubule-associated proteins tau, MAP2, and MAP4 on their microtubule-binding domain, causing their dissociation from microtubules and increased microtubule dynamics. Overexpression of MARK1 or MARK2 in cells leads to hyperphosphorylation of MAPs and to disruption of the microtubule array, resulting in morphological changes and cell death.
GenBank AF154845. The recombinant protein contains the amino acid substitutions V16E and T20S with reference to AF154845. These mutations are reported inGenBank NM_018650, AK098103 and BC068608.
Target class
Kinase
Family
CAMK
Accession number
NM_018650.2
Target Name
MARK1, active
Target Alias
MARK1, KIAA1477, MGC126512, MGC126513, MARK
Origin
Human
Theori. MW
92.7 kDa
Affinity tag
6His
Product specifications
Expression system
Expressed by baculovirus in Sf21 insect cells
Purity
Refer to CoA for Purity
Purification method
Ni2+/NTA-agarose
Sample Buffer
Specified activity
Refer to CoA
Application
For Research Only
Storage conditions
6 months at -70°C
Usage disclaimer
For Research Only
Chemical data
Background
MARK (MAP/microtubule affinity-regulating kinase) phosphorylates the microtubule-associated proteins tau, MAP2, and MAP4 on their microtubule-binding domain, causing their dissociation from microtubules and increased microtubule dynamics. Overexpression of MARK1 or MARK2 in cells leads to hyperphosphorylation of MAPs and to disruption of the microtubule array, resulting in morphological changes and cell death.
GenBank AF154845. The recombinant protein contains the amino acid substitutions V16E and T20S with reference to AF154845. These mutations are reported inGenBank NM_018650, AK098103 and BC068608.
Compound name
Kinase
Catalog number
14-651
Molecular formula
CAS
MW
Ka
Percent composition
Product specifications
Physical state
Purity (HPLC 214nm)
Retention time (RP18 HPLC)
CMC
Exact mass
Stability
For Research Only
Solubility structure
Datasheets
14-651
Datasheet